Identification of the novel HLA-DPB1 * 5801 allele detected by sequenced based typing
| Autor: | Marcel G.J. Tilanus, Anne Wil van der Zwan, Marapia A. Degli-Esposti, Roger L. Dawkins, L.F. Versluis, Louise N. Daly |
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| Rok vydání: | 1995 |
| Předmět: |
Genetics
HLA-DP Antigens Heterozygote Base Sequence HLA-DPB1 Histocompatibility Testing Molecular Sequence Data Immunology Exons Sequence Analysis DNA Biology DNA sequencing law.invention Histocompatibility law Humans Multilocus sequence typing Typing Allele Alleles HLA-DP beta-Chains Polymerase chain reaction |
| Zdroj: | Immunogenetics. 41:173-173 |
| ISSN: | 1432-1211 0093-7711 |
| DOI: | 10.1007/bf00182337 |
| Popis: | Within the framework of HLA-DPB typing of the Fourth Asia-Oceanic Histocompatibility workshop (4AOH) we have typed the A, B, and E panels representing 101 samples. Sequenced based typing (SBT) was used as the method for typing described by Versluis and co-workers, but the sequencing chemistry was modified; in this study we have used the Sequenase enzyme instead of the thermal stable Taq enzyme. Sequences obtained were compared to a database containing all known 55 HLA-DPB1 alleles. One sample showed a new heterozygous sequence, indicating the presence of a new allele. 4 refs., 1 fig. |
| Databáze: | OpenAIRE |
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