Parvovirus genome: nucleotide sequence of H-1 and mapping of its genes by hybrid-arrested translation
| Autor: | P R Paradiso, S L Rhode rd |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Genetics
Base Sequence Genes Viral Parvovirus viruses Immunology Nucleic acid sequence Biology biology.organism_classification Microbiology Genome Parvoviridae Open reading frame Viral Proteins Capsid Virology Insect Science Protein Biosynthesis Protein biosynthesis Amino Acids Gene Sequence (medicine) Research Article |
| Zdroj: | Journal of virology. 45(1) |
| ISSN: | 0022-538X |
| Popis: | The nucleotide sequence of the parvovirus H-1 has been determined by the chain-terminating method of Sanger. The sequence is 5,176 nucleotides long. Two large open reading frames (1 and 2) and two smaller open reading frames (3 and 4) of potential importance were identified in the plus-strand sequence. Promoter sequences are located at map positions 4 and 38 when map positions are expressed as percent of genome length from the 3' end of the virion minus strand. The locations for the genes for the parvovirus capsid proteins and a 76,000-dalton noncapsid protein (NCVP1) were mapped by hybrid-arrested translation. The gene for the capsid proteins VP1 and VP2' is located in the 5' half of the virus genome. The gene for NCVP1 is located in the 3' half of the viral DNA. |
| Databáze: | OpenAIRE |
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