Circulating microRNA-92a and microRNA-21 as novel minimally invasive biomarkers for primary breast cancer
| Autor: | Hai-yan Si, Huanchun Wang, Xiaoming Sun, Chengjin Hu, Yuan Cao, Shimin Chen, Yingjian Chen |
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| Rok vydání: | 2012 |
| Předmět: |
Oncology
CA15-3 KLK5 Cancer Research medicine.medical_specialty Gene Expression Breast Neoplasms Biology Breast cancer Internal medicine microRNA Biomarkers Tumor medicine Humans Neoplasm Invasiveness skin and connective tissue diseases Original Paper Hematology Cancer qRT-PCR General Medicine medicine.disease MicroRNAs Circulating MicroRNA Real-time polymerase chain reaction ROC Curve Case-Control Studies miR-92a Female miR-21 |
| Zdroj: | Journal of Cancer Research and Clinical Oncology |
| ISSN: | 1432-1335 0171-5216 |
| DOI: | 10.1007/s00432-012-1315-y |
| Popis: | Purpose MicroRNAs (miRNAs) play an essential role in breast malignant tumor development and progression. The development of clinically validated biomarkers for primary breast cancer (BC) has remained an insurmountable task despite other advances in the field of cancer molecular biology. The objective of this study is to investigate the differential expression of miRNAs and the potential of circulating microRNAs as novel primary breast cancer biomarkers. Methods Our analyses were performed on 48 tissue and 100 serum samples of patients with primary BC and a set of 20 control samples of healthy women, respectively. The relative expression of ten candidate miRNAs (miR-106b, miR-125b, miR-17, miR-185, miR-21, miR-558, miR-625, miR-665, miR-92a, and miR-93) from the results of four bioinformatics approaches and literature curation was measured by real-time quantitative reverse transcription PCR (qRT-PCR). Results The level of miR-92a was significantly lower, while miR-21 was higher, as previous reports, in tissue and serum samples of BC than that of healthy controls (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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