Long-term in vivo imaging of β-amyloid plaque appearance and growth in a mouse model of cerebral β-amyloidosis
| Autor: | Daniel Eicke, Michael E. Calhoun, Jasmin K. Hefendehl, David Milford, Mathias Jucker, Shinichi Kohsaka, Bettina M. Wegenast-Braun, Martin Eichner, Christian Liebig |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Genetically modified mouse
Male Pathology medicine.medical_specialty Amyloid Green Fluorescent Proteins metabolism [Amyloid beta-Peptides] Plaque Amyloid Mice Transgenic Lesion Mice pathology [Brain] Parenchyma Extracellular Medicine Animals genetics [Green Fluorescent Proteins] Humans Gliosis ddc:610 pathology [Plaque Amyloid] Predictive marker Amyloid beta-Peptides Staining and Labeling business.industry General Neuroscience Amyloidosis Brain pathology [Microglia] Articles pathology [Gliosis] medicine.disease Microscopy Fluorescence Multiphoton Female Microglia pathology [Amyloidosis] medicine.symptom business Preclinical imaging |
| Zdroj: | The journal of neuroscience 31(2), 624-629 (2011). doi:10.1523/JNEUROSCI.5147-10.2011 |
| DOI: | 10.1523/JNEUROSCI.5147-10.2011 |
| Popis: | Extracellular deposition of the amyloid-β peptide (Aβ) in the brain parenchyma is a hallmark lesion of Alzheimer's disease (AD) and a predictive marker for the progression of preclinical to symptomatic AD. Here, we used multiphotonin vivoimaging to study Aβ plaque formation in the brains of 3- to 4-month-old APPPS1 transgenic mice over a period of 6 months. A novel head fixation system provided robust and efficient long-term tracking of single plaques over time. Results revealed an estimated rate of 35 newly formed plaques per cubic millimeter of neocortical volume per week at 4–5 months of age. At later time points (i.e., in the presence of increasing cerebral β-amyloidosis), the number of newly formed plaques decreased. On average, both newly formed and existing plaques grew at a similar growth rate of 0.3 μm (radius) per week. A solid knowledge of the dynamics of cerebral β-amyloidosis in mouse models provides a powerful tool to monitor preclinical Aβ targeting therapeutic strategies and eases the interpretation of diagnostic amyloid imaging in humans. |
| Databáze: | OpenAIRE |
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