Two phenotypically and functionally distinct microglial populations in adult zebrafish
| Autor: | Wu, Shuting, Nguyen, Linh T. M., Pan, Hongru, Hassan, Shaoli, Dai, Yimei, Xu, Jin, Wen, Zilong |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
animal structures
Phagocytosis Central nervous system Population Mice 03 medical and health sciences 0302 clinical medicine Developmental Neuroscience medicine Animals education Zebrafish Research Articles Neuroinflammation 030304 developmental biology 0303 health sciences education.field_of_study Multidisciplinary Microglia biology fungi SciAdv r-articles biology.organism_classification White Matter Phenotype Cell biology medicine.anatomical_structure nervous system embryonic structures Neural development 030217 neurology & neurosurgery Research Article |
| Zdroj: | Science Advances |
| ISSN: | 2375-2548 |
| DOI: | 10.1126/sciadv.abd1160 |
| Popis: | Distinct microglial populations, phagocytotic microglia and regulatory microglia, are identified and characterized in zebrafish. Microglia are the tissue-resident macrophages in the central nervous system and are critically involved in immune defense, neural development and function, and neuroinflammation. The versatility of microglia has long been attributed to heterogeneity. Recent studies have revealed possible heterogeneity in human but not in murine microglia, yet a firm demonstration linking microglial heterogeneity to functional phenotypes remains scarce. Here, we identified two distinct microglial populations in adult zebrafish that differ in morphology, distribution, development, and function. The predominant population, phagocytotic microglia, which expresses ccl34b.1, is broadly distributed, amoeboid in shape, highly mobile, and phagocytotic. The other white matter–enriched ccl34b.1− population, regulatory microglia, has ramified protrusions but has limited mobility and phagocytosis capability. These functional differences are further supported by distinct transcriptomes and responses to bacterial infection, where ccl34b.1+ microglia function in tissue clearance and ccl34b.1− microglia release immune regulators. Our study sheds light on the heterogeneity and functional diversification of microglia. |
| Databáze: | OpenAIRE |
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