Subclinical cutaneous inflammation remained after permeability barrier disruption enhances UV sensitivity by altering ER stress responses and topical pseudoceramide prevents them
Autor: | Ji Hwoon Baek, Yutaka Takagi, Han Jo Kim, Seung Hun Lee, Sang Eun Lee, Takahiro Nishizaka |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Adult Keratinocytes medicine.medical_specialty XBP1 Erythema Ultraviolet Rays Interleukin-1beta Inflammation Human skin Dermatology CHOP Proinflammatory cytokine Tight Junctions 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine medicine Humans RNA Messenger skin and connective tissue diseases Skin integumentary system Chemistry Interleukin-6 Tumor Necrosis Factor-alpha General Medicine Endoplasmic Reticulum Stress 030104 developmental biology Photoprotection Unfolded protein response Cancer research CCAAT-Enhancer-Binding Proteins Unfolded Protein Response Tyrosine Female Spermine medicine.symptom |
Zdroj: | Archives of dermatological research. 309(7) |
ISSN: | 1432-069X |
Popis: | Stratum corneum forms the UV barrier. The effect of ultraviolet B (UVB) on normal skin was extensively studied; however, its effect on barrier perturbed skin remains undefined. Both barrier perturbation and UVB irradiation induce endoplasmic reticulum (ER) stress and unfolded protein response (UPR) in keratinocytes. Mild ER stress activates homeostatic UPR, while severe ER stress leads to abnormal UPR, promoting apoptosis and inflammation. Here, we investigated UV sensitivity and UVB-induced UPR in barrier-disrupted human skin and the effects of pseudoceramide-dominant emollient on UVB-induced skin responses. Tape-stripped skin of healthy volunteers showed enhanced susceptibility to erythema and augmented proinflammatory cytokines induction following suberythemal UVB irradiation. Suberythemal UVB activated XBP1 in normal skin, while increased CHOP transcription in barrier perturbed skin. After tape stripping, pseudoceramide-dominant emollient was applied for 3 days, and then, the areas were irradiated with suberythemal UVB. Pretreatment with topical pseudoceramide protected against UVB-induced upregulation of IL-1β, IL-6, and TNF-α transcription and reduced susceptibility to erythema following UVB. Topical pseudoceramide also suppressed suberythemal UVB-induced CHOP transcription in barrier-disrupted skin. Taken together, these data indicate that permeability barrier disruption increases UV sensitivity in human skin, partly via switch the UVB-induced UPR, from homeostatic signals to pro-apoptotic and proinflammatory signals. In addition, we conclude that pseudoceramide-dominant emollient suppresses excessive ER stress induction and CHOP activation following UVB in barrier damaged skin, providing evidence that pseudoceramide-dominant emollients can be promising strategies for photoprotection of the barrier damaged skin. |
Databáze: | OpenAIRE |
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