Choroidal lymphoma diagnosed by polymerase chain reaction-based clonality assessment
| Autor: | Miguel Fernández-Zarzoso, Mara Albert-Fort, Antonio Hernández-Pons, Elena Gómez-Beltrán, Lucía Martínez-Costa |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty Lymphoma B-Cell Polymerase Chain Reaction law.invention 03 medical and health sciences 0302 clinical medicine Antineoplastic Agents Immunological law medicine Humans Polymerase chain reaction Ultrasonography business.industry Choroid Neoplasms General Medicine DNA Neoplasm Middle Aged medicine.disease Lymphoma Ophthalmology 030220 oncology & carcinogenesis Intravitreal Injections 030221 ophthalmology & optometry Female Intraocular lymphoma business Immunoglobulin Heavy Chains Rituximab Nucleic Acid Amplification Techniques |
| Zdroj: | European journal of ophthalmology. 31(2) |
| ISSN: | 1724-6016 |
| Popis: | Purpose: To report a case of primary choroidal lymphoma that was confirmed by polymerase chain reaction–based clonality testing. Case report: A 50-year-old woman presented with unilateral progressive vision loss. Fundus examination and B-ultrasonography demonstrated diffuse choroidal thickening without vitritis. Pars plana vitrectomy and subretinal biopsy were performed, and histopathologic analysis revealed choroidal B-cell lymphoid hyperplasia without evidence of neoplasia. Extraocular extension was ruled out, and transitory improvement was observed with oral steroids. After 1-year follow-up, she was referred to our hospital and clonality testing was performed using the samples taken months before. First, we used a forensic DNA extraction kit, and then, a multiplex polymerase chain reaction was carried out using the IgH Rearrangements Molecular Analysis Kit. Clonal rearrangement was identified for the immunoglobulin heavy chain framework regions 1 and 2, and B-cell choroidal lymphoma was confirmed. The patient began treatment with intravitreal rituximab, but no response was observed. Finally, complete regression was achieved using external beam radiotherapy. Conclusion: Polymerase chain reaction–based clonality testing can be a valuable tool to confirm a choroidal lymphoproliferative process. |
| Databáze: | OpenAIRE |
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