Comparison of a novel cholesterol efflux assay using immobilized liposome-bound gel beads with the conventional method
| Autor: | Yuna Horiuchi, Takahiro Kameda, Minoru Tozuka, Ryunosuke Ohkawa, Shao-Jui Lai |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Apolipoprotein B THP-1 Cells Porphobilinogen Biophysics high-density lipoprotein 030204 cardiovascular system & hematology Biochemistry 03 medical and health sciences chemistry.chemical_compound Apolipoproteins E 0302 clinical medicine High-density lipoprotein apolipoproteinA-I Humans immobilized liposome-bound gel beads Molecular Biology Diagnostics & Biomarkers Research Articles Reproducibility Liposome Chromatography Apolipoprotein A-I biology Chemistry Cholesterol Cholesterol HDL Reproducibility of Results Biological Transport Cell Biology Repeatability cholesterol efflux capacity Metabolism 030104 developmental biology Cardiovascular System & Vascular Biology Apolipoprotein B-100 Liposomes cardiovascular system biology.protein Biological Assay lipids (amino acids peptides and proteins) Efflux ATP Binding Cassette Transporter 1 Foam Cells Lipoprotein |
| Zdroj: | Bioscience Reports |
| ISSN: | 1573-4935 0144-8463 |
| Popis: | Cholesterol efflux capacity (CEC) is an atheroprotective function of high-density lipoprotein (HDL). CEC is currently measured using artificially prepared foam cells composed of cultured macrophage and 3H-cholesterol. However, this conventional method is not suitable for clinical laboratory use due to poor repeatability, complexity, and low safety. Recently, we reported a novel CEC assay, called the immobilized liposome-bound gel beads (ILG) method. The ILG method is an alternative to foam cells, comprising gel beads and 4,4-diflioro-4-bora-3a,4a-s-indacene labeled cholesterol (BODIPY-cholesterol) instead of macrophage and 3H-cholesterol, respectively. The ILG method has shown adequate basic properties and strong correlation with the conventional method. Here, we aimed to compare this new ILG method with the conventional method in-depth. When apoB-depleted serum was used as the cholesterol acceptor (CA), the ILG method had far better reproducibility than the conventional method. The CEC of major HDL subclasses HDL2 and HDL3 had similar results in both the ILG and conventional method. However, the ILG method did not reflect the CEC of apolipoprotein (apo) A–I and a minor HDL subclass which uses ATP-binding cassette transporter A1 on foam cells. Superior reproducibility of the ILG method, which is a limitation of the conventional method, and similar CEC results for major HDL subclasses in the ILG and conventional methods, provide further evidence that the ILG method is promising for measuring CEC clinically. However, some HDL subclasses or apo might have poor CEC correlation between these methods. Further research is therefore needed to confirm the clinical significance of estimating CEC by the ILG method. |
| Databáze: | OpenAIRE |
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