Screening of DNA Signaling Aptamer from Multiple Candidates Obtained from SELEX with Next-generation Sequencing
| Autor: | Keiko Kimura, Koji Wakui, Keitaro Yoshimoto, Hitoshi Furusho, Toru Yoshitomi, Fumiya Wayama |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
VEGF receptors
Aptamer 02 engineering and technology Computational biology G-quadruplex 01 natural sciences DNA sequencing Analytical Chemistry chemistry.chemical_compound Gene Library Vascular Endothelial Growth Factor Receptor-1 Base Sequence biology Chemistry SELEX Aptamer Technique 010401 analytical chemistry High-Throughput Nucleotide Sequencing Aptamers Nucleotide 021001 nanoscience & nanotechnology Vascular Endothelial Growth Factor Receptor-2 0104 chemical sciences biology.protein 0210 nano-technology Systematic evolution of ligands by exponential enrichment DNA Protein Binding |
| Zdroj: | Analytical Sciences. 35:113-116 |
| ISSN: | 1348-2246 0910-6340 |
| Popis: | Here, we demonstrated a strategy for developing signaling aptamers, based on screening of signaling aptamers from multiple aptamer candidates obtained by SELEX with next generation sequencing. Among aptamer candidates labelled by 6-carboxyfluorescein and quencher at both end termini, there is the possibility of discovering a potent signaling aptamer. In this study, we discovered DNA signaling aptamers against VEGFR-1. This strategy has the potential for signaling aptamer discovery without the extremely laborious task of optimization of oligodeoxynucleotide modifications. |
| Databáze: | OpenAIRE |
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