Advanced glycosylation end products inhibit the proliferation of bone-marrow stromal cells through activating MAPK pathway
| Autor: | Meng Wan, Zheng Li, Zhan-Yi Gao, Hao-Jie Wang, Xiao Wang, Tian-Pei Hong |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
MAPK/ERK pathway
Glycation End Products Advanced Male Stromal cell MAP Kinase Kinase 4 MAP Kinase Signaling System p38 mitogen-activated protein kinases Proliferation Bone Marrow Cells p38 Mitogen-Activated Protein Kinases Rats Sprague-Dawley Animals Viability assay Protein kinase A Protein kinase B Advanced glycosylation end products PI3K/AKT/mTOR pathway Cells Cultured Cell Proliferation Chemistry Research NF-kappa B Bone-marrow stromal cells Mesenchymal Stem Cells MAPK pathway General Medicine Molecular biology Rats Medicine Signal transduction |
| Zdroj: | European Journal of Medical Research, Vol 26, Iss 1, Pp 1-11 (2021) European Journal of Medical Research |
| Popis: | Background The purpose of present study was to explore the mechanism of nuclear factor-kappa B (NF-κB), phosphatidylinositol 3-kinase (PI3K)/protein kinase B(PKB/Akt) and mitogen-activated protein kinase (MAPK) signaling pathways after intervention of advanced glycosylation end products (AGEs) on rat bone-marrow stromal cells (BMSCs). Methods Prepare and identify AGEs. BMSCs were isolated from 16 SD rats and cultured with different concentration of AGEs. Cell viability was detected by cell counting kit-8 (CCK-8). BMSCs were cultured with AGEs (0.25 mg/ml) for 30 min, 12 h, 24 h, 72 h and 120 h. In addition, BMSCs were cultured with AGEs, AGEs + JNK inhibitor and AGEs + P38 inhibitor for 24 h and 48 h, respectively. Western blotting and RT-PCR were used to determine the protein and mRNA expression levels, respectively. Results Cell viability of BMSCs was significantly correlated with concentration and effect time of AGEs (P P P P > 0.05). At the mRNA level, JNK and p38 inhibitors significantly reduced the levels of NF-κB p65, p38 and JNK, increased IκB (P > 0.05), but had no effect on Akt in BMSCs (P > 0.05). At the protein level, JNK and p38 inhibitors notably decreased the expression of NF-κB p65, p38, p-JNK, P-IκB and JNK (P P Conclusion Advanced glycosylation end products can inhibit the proliferation of bone-marrow stromal cells through activating MAPK pathway. |
| Databáze: | OpenAIRE |
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