Angiotensin II Regulates microRNA-132/-212 in Hypertensive Rats and Humans

Autor: Maria Lyck Hansen, Anne Yaël Nossent, Mikael Schneider, Lars Melholt Rasmussen, Pia Jeppesen, Pernille B. Lærkegaard Hansen, Tilde Eskildsen, Maria B. Sandberg, Charlotte Harken Jensen, Ditte Caroline Andersen, Søren P. Sheikh, Niels Marcussen, Peter Bie
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Angiotensin receptor
AT1R
AT1 receptor blocker
Blood Pressure
AT(1) receptor blocker
AT1R
Rats
Sprague-Dawley
lcsh:Chemistry
Mice
Vasoconstrictor Agents
Receptor
lcsh:QH301-705.5
Spectroscopy
Oligonucleotide Array Sequence Analysis
Endothelin-1
microRNA
Angiotensin II
AT1 receptor blocker
General Medicine
Computer Science Applications
Organ Specificity
Female
Endothelin receptor
medicine.medical_specialty
hypertension
Cardiomegaly
Biology
Catalysis
Article
Inorganic Chemistry
Internal medicine
medicine
Animals
Humans
Physical and Theoretical Chemistry
Molecular Biology
Organic Chemistry
Reproducibility of Results
medicine.disease
Fibrosis
Endothelin 1
Mice
Inbred C57BL
Disease Models
Animal
MicroRNAs
Blood pressure
Endocrinology
Gene Expression Regulation
lcsh:Biology (General)
lcsh:QD1-999
Heart failure
Pathophysiology of hypertension
AT(1)R
Angiotensin II Type 1 Receptor Blockers
Zdroj: International Journal of Molecular Sciences, Vol 14, Iss 6, Pp 11190-11207 (2013)
International Journal of Molecular Sciences
International Journal of Molecular Sciences, 14(6), 11190-11207
Eskildsen, T V, Jeppesen, P L, Schneider, M, Nossent, A Y, Sandberg, M B, Hansen, P B L, Jensen, C H, Hansen, M L, Marcussen, N, Rasmussen, L M, Bie, P, Andersen, D C & Sheikh, S P 2013, ' Angiotensin II Regulates microRNA-132/-212 in Hypertensive Rats and Humans ', International Journal of Molecular Sciences, vol. 14, no. 6, pp. 11190-11207 . https://doi.org/10.3390/ijms140611190
International Journal of Molecular Sciences; Volume 14; Issue 6; Pages: 11190-11207
ISSN: 1422-0067
Popis: MicroRNAs (miRNAs), a group of small non-coding RNAs that fine tune translation of multiple target mRNAs, are emerging as key regulators in cardiovascular development and disease. MiRNAs are involved in cardiac hypertrophy, heart failure and remodeling following cardiac infarction; however, miRNAs involved in hypertension have not been thoroughly investigated. We have recently reported that specific miRNAs play an integral role in Angiotensin II receptor (AT1R) signaling, especially after activation of the Gαq signaling pathway. Since AT1R blockers are widely used to treat hypertension, we undertook a detailed analysis of potential miRNAs involved in Angiotensin II (AngII) mediated hypertension in rats and hypertensive patients, using miRNA microarray and qPCR analysis. The miR-132 and miR-212 are highly increased in the heart, aortic wall and kidney of rats with hypertension (159 ± 12 mm Hg) and cardiac hypertrophy following chronic AngII infusion. In addition, activation of the endothelin receptor, another Gαq coupled receptor, also increased miR-132 and miR-212. We sought to extend these observations using human samples by reasoning that AT1R blockers may decrease miR-132 and miR-212. We analyzed tissue samples of mammary artery obtained from surplus arterial tissue after coronary bypass operations. Indeed, we found a decrease in expression levels of miR-132 and miR-212 in human arteries from bypass-operated patients treated with AT1R blockers, whereas treatment with β-blockers had no effect. Taken together, these data suggest that miR-132 and miR-212 are involved in AngII induced hypertension, providing a new perspective in hypertensive disease mechanisms.
Databáze: OpenAIRE
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