Comparative Analysis of the Different Dyes’ Potential to Assess Human Normal and Cancer Cell Viability In Vitro under Different D/H Ratios in a Culture Medium
| Autor: | S. N. Novikova, S. A. Dolenko, Olga Gubar, R. G. Vasyliev, Anton Syroeshkin, Igor Zlatskiy, Alona Zlatska, D. A. Zubov, I M Gordiienko, Nadine Antipova |
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| Rok vydání: | 2020 |
| Předmět: |
Technology
Cell type Article Subject Science General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Viability assay 030304 developmental biology General Environmental Science 0303 health sciences Growth medium Chemistry Cancer General Medicine medicine.disease Molecular biology In vitro Cancer cell Medicine 030217 neurology & neurosurgery |
| Zdroj: | The Scientific World Journal, Vol 2020 (2020) |
| ISSN: | 1537-744X 2356-6140 |
| Popis: | In this study, using new approach (laser diffraction + biological dyes), we have demonstrated the decrease of cells viability in vitro in the deuterated growth medium, whereas in the deuterium-depleted medium, there was an increase of cell viability. We have also found that not all dyes are equally sensitive to the D/H ratios in the culture medium (system) as well as to the different cell types (cancer vs normal cells). |
| Databáze: | OpenAIRE |
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