Improving selenium extraction by sequential enzymatic processes for Se-speciation of selenium-enriched Agaricus bisporus
| Autor: | Zs. Stefánka, Mihály Dernovics, Péter Fodor |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Mushroom
Lysis Chromatography Glycoside Hydrolases Agaricus Extraction (chemistry) chemistry.chemical_element Pronase Biochemistry Analytical Chemistry Fungal Proteins Spectrometry Fluorescence chemistry Cell Wall Endopeptidases Sample preparation Selenium Compounds Digestion Selenium Agaricus bisporus |
| Zdroj: | Analytical and Bioanalytical Chemistry. 372:473-480 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-001-1215-5 |
| Popis: | Sample preparation methods based on the use of proteolytic and cell wall digesting enzymes for the speciation analysis of selenized mushroom were investigated. The sample (Agaricus bisporus; 160 microg total Se per g sample) was grown on compost supplemented with selenized yeast. Experiments were carried out to elucidate the possible role of the cell wall digesting enzymes--Lysing enzyme and Driselase--in the improvement of extraction efficiency with and without inhibiting proteolysis during cell wall digestion. A 3-step procedure applying Lysing enzyme and pronase gave the highest extraction efficiency (89%); however, the best species recovery was achieved by a one-step proteolytic procedure. All the procedures of selenium speciation were controlled by independent ICP-AES analysis measuring the total amount of selenium. |
| Databáze: | OpenAIRE |
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