Effect of ret/PTC 1 rearrangement on transcription and post-transcriptional regulation in a papillary thyroid carcinoma model
| Autor: | Richard Henfrey, Richard Flavin, Esther O'Regan, John J. O'Leary, Simone M Guenther, Astrid Potratz, Susanne Cahill, K Denning, J Li, Orla Sheils, Paul Smyth, Stephen P. Finn |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Cancer Research
Oncogene Proteins Fusion Transcription Genetic endocrine system diseases Biology lcsh:RC254-282 Thyroid carcinoma Cell Line Tumor microRNA Biomarkers Tumor Humans RNA Messenger Thyroid Neoplasms RNA Processing Post-Transcriptional Kinase activity Post-transcriptional regulation Oligonucleotide Array Sequence Analysis Gene Rearrangement Oncogene Reverse Transcriptase Polymerase Chain Reaction Research Gene Expression Profiling Gene rearrangement Protein-Tyrosine Kinases lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Molecular biology Carcinoma Papillary Gene expression profiling MicroRNAs Oncology Cancer research Molecular Medicine DNA microarray |
| Zdroj: | Molecular Cancer, Vol 5, Iss 1, p 70 (2006) Molecular Cancer |
| ISSN: | 1476-4598 |
| Popis: | BackgroundmicroRNAs (miRNAs) are a group of non-coding single stranded RNAs measuring approximately 22 nt in length that have been found to control cell growth, differentiation and apoptosis. miRNAs negatively regulate their target genes and recently have been implicated in tumourigenesis. Furthermore, miRNA expression profiling correlates with various cancers, with these genes thought to act as both tumour suppressors and oncogenes. ret/PTC 1 is an oncogene with constitutive kinase activity implicated in the development of papillary thyroid carcinoma (PTC). This rearrangement leads to aberrant MAPK activation that is implicated in PTC tumourigenesis.AimThe aim of this study was to identify the effect that ret/PTC 1 has on transcription and post-transcriptional regulation in PTC by using DNA microarray and microRNA analysis.ResultsDNA microarray analysis revealed a group of genes differentially expressed between normal thyroid cell lines and those harbouring a ret/PTC 1 rearrangement.Furthermore, a unique miRNA expression signature differentiated between PTC cell lines with ret/PTC 1 and a normal thyroid cell line. 21 miRNAs showed significant overexpression and 14 miRNAs showed underexpression in these cell lines when compared to normal thyroid. Several of these up/down regulated miRNAs may be involved in PTC pathogenesis. |
| Databáze: | OpenAIRE |
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