Zearalenone nephrotoxicity: DNA fragmentation, apoptotic gene expression and oxidative stress protected by Lactobacillus plantarum MON03
| Autor: | Khawla Ezzdini, Hela Belgacem, Mosaad A. Abdel-Wahhab, Samir Abbès, Jalila Ben Salah-Abbès |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Gene Expression DNA Fragmentation Kidney Toxicology medicine.disease_cause 01 natural sciences Nephrotoxicity Andrology 03 medical and health sciences chemistry.chemical_compound Malondialdehyde Gene expression medicine Animals Estrogens Non-Steroidal chemistry.chemical_classification Glutathione Peroxidase Mice Inbred BALB C 0303 health sciences biology 010604 marine biology & hydrobiology Glutathione peroxidase fungi 030302 biochemistry & molecular biology biology.organism_classification Oxidative Stress medicine.anatomical_structure chemistry Zearalenone DNA fragmentation Lactobacillus plantarum Oxidative stress |
| Zdroj: | Toxicon. 175:28-35 |
| ISSN: | 0041-0101 |
| DOI: | 10.1016/j.toxicon.2019.12.004 |
| Popis: | The present study was conducted to determine the abilities of the living Lactobacillus plantarum MON03 cells to degrade Zearalenone (ZEN) in liquid medium, and to elucidate the preventive effect in ZEN-contaminated balb/c mice showing kidney damage. The DNA fragmentation, Bcl-2 and Bax gene expression, caspase-3 activity, mRNA level of inflammation-regulating cytokines and histology of kidney tissues were examined. Female Balb/c mice were divided into four groups (10/group) and treated daily for 2 wk by oral gavage with lactic acid bacteria (L. plantarum MON03) 2 × 109 CFU/L, ~2 mg/kg only, ZEN (40 mg/kg BW) only, ZEN (40 mg/kg BW) + lactic acid bacteria (L. plantarum MON03, 2 × 109 CFU/L, ~2 mg/kg). Control group received vehicle. At the end of experiment, the kidney was collected for the determination of DNA fragmentation, Bcl-2 and Bax gene expression,caspase-3 activity, Malondialdehyde (MDA), and glutathione peroxidase (GSH-Px) content, as well as for any alterations in expression of total antioxidant activity (TAC) and mRNA levels of inflammation-regulating cytokines (e.g., IL-10, IL-6, TNF-alpha). The results indicated that, kidney cells exposure to ZEN led to increased caspase-3 activity, MDA, and IL-10, IL-6, TNF-alpha and Bax mRNA levels, but decreased TAC content and down-regulated expression of GSH-Px and CAT and Bcl-2 mRNA. Co-treatment with ZEN plus LP suppressed the levels of DNA fragmentation; normalized kidney MDA and increased CAT levels, up-regulated expression of GSH-Px and CAT, and normalized mRNA levels of the analyzed cytokines. It's concluded that ZEN might have toxic effects in kidney. Further, it can be seen that use of LP induced protective effects against the oxidative stress and kidney toxicity of ZEN in part through adhesion (and so likely diminished bioavailability). |
| Databáze: | OpenAIRE |
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