Galleria mellonella as an infection model for Campylobacter jejuni virulence
| Autor: | Mary C. Bagnall, Olivia L. Champion, Nicola J. Senior, Francisco J. Salguero, Richard W. Titball, Martin J. Woodward, Stuart E. Reynolds, Roberto M. La Ragione |
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| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
Hemocytes animal structures Virulence Moths Spodoptera Microbiology Campylobacter jejuni Cell Line Lepidoptera genitalia Mice Campylobacter Jejuni Infection Campylobacter Infections Extracellular Animals Humans biology Macrophages fungi General Medicine bacterial infections and mycoses biology.organism_classification Virology Bacterial Typing Techniques Galleria mellonella Disease Models Animal Larva Multilocus sequence typing Bacteria Multilocus Sequence Typing |
| Zdroj: | Senior, N J, Bagnall, M C, Champion, O L, Reynolds, S E, La Ragione, R M, Woodward, M J, Salguero, F J & Titball, R W 2011, ' Galleria mellonella as an infection model for Campylobacter jejuni virulence ', Journal of Medical Microbiology, vol. 60, no. 5, pp. 661-669 . https://doi.org/10.1099/jmm.0.026658-0 |
| ISSN: | 1473-5644 0022-2615 |
| Popis: | Larvae of Galleria mellonella (Greater Wax Moth) have been shown to be susceptible to Campylobacter jejuni infection and our study characterizes this infection model. Following infection with C. jejuni human isolates, bacteria were visible in the haemocoel and gut of challenged larvae, and there was extensive damage to the gut. Bacteria were found in the extracellular and cell-associated fraction in the haemocoel, and it was shown that C. jejuni can survive in insect cells. Finally, we have used the model to screen a further 67 C. jejuni isolates belonging to different MLST types. Isolates belonging to ST257 were the most virulent in the Galleria model, whereas those belonging to ST21 were the least virulent. |
| Databáze: | OpenAIRE |
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