Quantitative Determination of Glycine in Aqueous Solution Using Glutamate Dehydrogenase-Immobilized Glyoxal Agarose Beads
| Autor: | Semra Yılmazer Keskin, Can Serkan Keskin |
|---|---|
| Přispěvatelé: | Keskin, SY, Keskin, CS, Sakarya Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü, Yılmazer Keskin, Semra, Keskin, Can Serkan |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Time Factors
Glycine Glutamate dehydrogenase Glyoxal agarose Indophenol blue FTIR Glutamic Acid Bioengineering Biosensing Techniques Applied Microbiology and Biotechnology Biochemistry Enzyme catalysis chemistry.chemical_compound Glutamate Dehydrogenase Fourier transform infrared spectroscopy Molecular Biology chemistry.chemical_classification Chromatography Aqueous solution Sepharose Temperature Glyoxylates Water General Medicine Hydrogen-Ion Concentration Enzymes Immobilized Microspheres Amino acid Solutions Indophenol chemistry Glyoxal Agarose Biotechnology |
| Popis: | In this study, an enzymatic procedure for the determination of glycine (Gly) was developed by using a column containing immobilized glutamate dehydrogenase (GDH) on glyoxal agarose beads. Ammonia is produced from the enzymatic reactions between Gly and GDH with NAD(+) in phosphate buffer medium. The indophenol blue method was used for ammonia detection based on the spectrophotometric measurements of blue-colored product absorbing at 640 nm. The calibration graph is linear in the range of 0.1-10 mM of Gly concentrations. The effect of pH, temperature, and time interval was studied to find column stability, and also the interference effects of other amino acids was investigated. The interaction between GDH and glyoxal agarose beads was analyzed by Fourier transform infrared (FTIR) spectroscopy. The morphology of the immobilized and non-immobilized agarose beads were characterized by atomic force microscopy (AFM). |
| Databáze: | OpenAIRE |
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