Analysis of the Mouse dhfr Promoter Region: Existence of a Divergently Transcribed Gene
| Autor: | G F Crouse, E G Frayne, Rodney E. Kellems, R N McEwan, E J Leys |
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| Rok vydání: | 1985 |
| Předmět: |
Amanitins
Transcription Genetic Ultraviolet Rays Base pair Mutant Biology Mice Exon Transcription (biology) parasitic diseases Gene duplication Dihydrofolate reductase Animals heterocyclic compounds Cloning Molecular Peptide Chain Initiation Translational Promoter Regions Genetic Molecular Biology Gene Genetics Base Sequence Single-Strand Specific DNA and RNA Endonucleases Gene Amplification Dose-Response Relationship Radiation Promoter Cell Biology Endonucleases Molecular biology Tetrahydrofolate Dehydrogenase enzymes and coenzymes (carbohydrates) Genes Mutation biology.protein Research Article |
| Zdroj: | Molecular and Cellular Biology. 5:1847-1858 |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.5.8.1847-1858.1985 |
| Popis: | The use of murine dihydrofolate reductase (dhfr) gene amplification mutants enabled us to identify important structural and functional features of the dhfr promoter region. We found another transcription unit, at least 14 kilobases in size, which initiates within 130 base pairs of the major dhfr transcript and is transcribed divergently. The 5' ends of both transcripts were analyzed and found to have multiple initiation sites. The major dhfr transcript and the divergent transcript appear to share the same promoter region; the longer transcripts of the dhfr gene overlap with the divergent transcripts and use a different promoter region. The divergent transcript appears to code for a protein; an homologous sequence to its first exon is found in the corresponding location near the human dhfr gene. |
| Databáze: | OpenAIRE |
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