MV-mimicking micelles loaded with PEG-serine-ACP nanoparticles to achieve biomimetic intra/extra fibrillar mineralization of collagen in vitro
| Autor: | Jingjing Deng, Mengqi Zhu, Wenxin Zhang, Xu Zhang, Danyang Lu, Mingli Lin, Minjuan Shen, Huanhuan Liu, Kehua Que |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Calcium Phosphates
Light Biophysics 02 engineering and technology Polyethylene glycol 010402 general chemistry 01 natural sciences Biochemistry Micelle Mineralization (biology) Bone and Bones Polyethylene Glycols chemistry.chemical_compound Calcification Physiologic X-Ray Diffraction In vivo Biomimetic Materials PEG ratio Serine Humans Scattering Radiation Amorphous calcium phosphate Molecular Biology Micelles Tissue Engineering Tissue Scaffolds Chemistry Vesicle Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology 0104 chemical sciences Extracellular Matrix Nanoparticles Collagen 0210 nano-technology Biomineralization |
| Zdroj: | Biochimica et biophysica acta. General subjects. 1863(1) |
| ISSN: | 1872-8006 |
| Popis: | Since their discovery, matrix vesicles (MVs) containing minerals have received considerable attention for their role in the mineralization of bone, dentin and calcified cartilage. Additionally, MVs' association with collagen fibrils, which serve as the scaffold for calcification in the organic matrix, has been repeatedly highlighted. The primary purpose of the present study was to establish a MVs-mimicking model (PEG-S-ACP/micelle) in vitro for studying the exact mechanism of MVs-mediated extra/intra fibrillar mineralization of collagen in vivo. In this study, high-concentration serine was used to stabilize the amorphous calcium phosphate (S-ACP), which was subsequently mixed with polyethylene glycol (PEG) to form PEG-S-ACP nanoparticles. The nanoparticles were loaded in the polysorbate 80 micelle through a micelle self-assembly process in an aqueous environment. This MVs-mimicking model is referred to as the PEG-S-ACP/micelle model. By adjusting the pH and surface tension of the PEG-S-ACP/micelle, two forms of minerals (crystalline mineral nodules and ACP nanoparticles) were released to achieve the extrafibrillar and intrafibrillar mineralization, respectively. This in vitro mineralization process reproduced the mineral nodules mediating in vivo extrafibrillar mineralization and provided key insights into a possible mechanism of biomineralization by which in vivo intrafibrillar mineralization could be induced by ACP nanoparticles released from MVs. Also, the PEG-S-ACP/micelle model provides a promising methodology to prepare mineralized collagen scaffolds for repairing bone defects in bone tissue engineering. |
| Databáze: | OpenAIRE |
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