Extracellular high mobility group box chromosomal protein 1 promotes drug resistance by increasing the expression of P-glycoprotein expression in gastric adenocarcinoma cells
Autor: | Yuping Yin, Wei Li, Meizhou Deng, Qian Shen, Guobing Wang, Peng Zhang, Kaixiong Tao |
---|---|
Rok vydání: | 2014 |
Předmět: |
Cancer Research
Blotting Western Cell Antineoplastic Agents Apoptosis chemical and pharmacologic phenomena Adenocarcinoma Biochemistry Stomach Neoplasms Cell Line Tumor Genetics Extracellular medicine Humans ATP Binding Cassette Transporter Subfamily B Member 1 HMGB1 Protein Molecular Biology P-glycoprotein biology Cell growth Cell cycle Molecular biology Gene Expression Regulation Neoplastic medicine.anatomical_structure Oncology Drug Resistance Neoplasm Cell culture Cancer cell biology.protein Cancer research Molecular Medicine Extracellular Space |
Zdroj: | Molecular Medicine Reports. 9:1439-1443 |
ISSN: | 1791-3004 1791-2997 |
Popis: | It has previously been reported that high mobility group box chromosomal protein 1 (HMGB1) is overexpressed in the majority of gastric adenocarcinoma cell types, and that HMGB1 can be released into the extracellular matrix from stressed or necrotic cancer cells. HMGB1 is considered to promote cell proliferation and invasion in gastric adenocarcinoma cells. Furthermore, in a number of cancer cell types, HMGB1 has been reported to promote autophagy and inhibit anticancer drug‑induced apoptosis, which has been identified as an important mechanism in the development of multidrug resistance (MDR). However, there have been no studies on the effects of HMGB1 on expression of the MDR‑related transporter proteins in gastric adenocarcinoma. In the present study, extracellular HMGB1 increased the expression levels of P-glycoprotein (P-gp) at the pre-transcriptional and post‑transcriptional levels in the human gastric adenocarcinoma cell lines, SGC7901, MKN28 and AGS, as detected by quantitative polymerase chain reaction and western blot assays. MTT and apoptosis assays were also performed and it was demonstrated that extracellular HMGB1 subsequently enhanced resistance to the P‑gp‑related drugs, adriamycin and vincristine. In brief, this study demonstrated that extracellular HMGB1 may promote drug resistance to adriamycin and vincristine by upregulating P‑gp in human gastric adenocarcinoma cells. |
Databáze: | OpenAIRE |
Externí odkaz: |