Expression of the high-affinity IgE receptor on peripheral blood dendritic cells: Differential binding of IgE in atopic asthma
| Autor: | Amanda E. Semper, Judith A. Holloway, Stephen T. Holgate |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Adult
Hypersensitivity Immediate Adolescent CD14 Immunology CD16 Immunoglobulin E CD19 Atopy Humans Immunology and Allergy Medicine Functional ability Antigen-presenting cell biology Receptors IgE business.industry Dendritic Cells Dendritic cell Middle Aged Flow Cytometry medicine.disease Asthma Leukocytes Mononuclear biology.protein business |
| Zdroj: | Journal of Allergy and Clinical Immunology. 107:1009-1018 |
| ISSN: | 0091-6749 |
| DOI: | 10.1067/mai.2001.115039 |
| Popis: | Background: Dendritic cells can express the high-affinity IgE receptor (FcϵRI), which, in the presence of specific IgE, facilitates the uptake of allergen, leading to increased activation of allergen-specific T cells. FcϵRI expression by dendritic cells is higher in the airways of atopic asthmatic subjects than in those of healthy, nonatopic control subjects. Objective: The aims of this study were to determine whether a similar difference in FcϵRI expression occurs between dendritic cells in the peripheral blood of atopic asthmatic subjects and healthy individuals and also whether an altered ability of FcϵRI + peripheral blood dendritic cells to bind IgE accompanies the atopic asthmatic state. Methods: Flow cytometry was used to analyze the surface expression of FcϵRI and exogenously bound IgE on dendritic cells identified as lineage negative (CD3, CD14, CD16, CD19, and CD56) and HLA-DR bright. Results: The total expression of FcϵRI on the surface of dendritic cells from healthy and asthmatic subjects was not significantly different. However, in vivo, dendritic cells from atopic asthmatic subjects had higher levels of receptor occupancy by IgE and bound exogenous IgE in vitro more efficiently than dendritic cells from healthy subjects. Conclusion: The similar levels of expression of FcϵRI on peripheral blood dendritic cells from healthy and asthmatic subjects suggest that the local environment in the airway is responsible for the upregulation of surface FcϵRI on airway dendritic cells in asthma. The results also suggest that the functional ability of FcϵRI to bind IgE is differentially controlled in the atopic state. (J Allergy Clin Immunol 2001;107:1009-18.) |
| Databáze: | OpenAIRE |
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