Study of the intra-arterial distribution of Fe3O4 nanoparticles in a model of colorectal neoplasm induced in rat liver by MRI and spectrometry
| Autor: | Alberto Saiz-López, Begoña Fernández-Ruanova, Nestor Etxebarria, Jose Javier Echevarría-Uraga, Ignacio García-Alonso, Fernando Plazaola, Maite Insausti |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
Pathology medicine.medical_specialty Materials science Biophysics Pharmaceutical Science Nanoparticle Bioengineering Mass spectrometry hepatic arterial infusion Mass Spectrometry Statistics Nonparametric Biomaterials spectrometry Hepatic arterial infusion Hepatic Artery International Journal of Nanomedicine Cell Line Tumor Drug Discovery medicine Intra arterial liver neoplasm Distribution (pharmacology) Animals Liver neoplasm Tissue Distribution Magnetite Nanoparticles Original Research iron concentration medicine.diagnostic_test Organic Chemistry Liver Neoplasms Magnetic resonance imaging General Medicine Magnetic Resonance Imaging Rats Liver Rat liver Injections Intravenous Colorectal Neoplasms ferromagnetic particle MRI |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 1176-9114 |
| Popis: | José J Echevarria-Uraga,1 Ignacio García-Alonso,2 Fernando Plazaola,3 Maite Insausti,3 Néstor Etxebarria,3 Alberto Saiz-López,4 Begoña Fernández-Ruanova51Radiology Department, Hospital de Galdakao-Usánsolo, Bizkaia, Spain; 2Experimental Surgery Laboratory, Medicine Faculty, University of the Basque Country, Bizkaia, Spain; 3Faculty of Science and Technology, University of the Basque Country, Bizkaia, Spain; 4Pathology Department, Hospital de Galdakao-Usánsolo, Bizkaia, Spain; 5Osatek SA Unidad del Hospital de Galdakao-Usánsolo, Bizkaia, SpainPurpose: To evaluate, in an experimental model, the reliability of MRI for determining whether a higher iron concentration was obtained in tumor tissue than in normal liver parenchyma after intra-arterial administration of Fe3O4 lipophilic nanoparticles.Materials and methods: WAG/RijCrl rats were inoculated in the left hepatic lobe with 25,000 syngeneic CC-531 colon adenocarcinoma cells, after which they were randomized into two groups: control (CG) and infused (IG). After confirming tumor induction, the IG rats received intra-arterial suspensions of Fe3O4 nanoparticles (2.6 mg) in Lipiodol® (0.15 mL). To calculate the iron concentration, [Fe], in the tumor and liver tissues of both groups of rats, measurements of signal intensity from the tumors, healthy liver tissue, and paravertebral muscles were made on a 1.5T MRI system in gradient-echo DP* and T2*-weighted sequences. In addition, samples were collected to quantify the [Fe] by inductively coupled plasma-mass spectrometry (ICP-MS), as well as for histological analysis. Statistical analysis was performed with non-parametric tests, and Bland–Altman plots were produced; P values |
| Databáze: | OpenAIRE |
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