The detailed physical map of the temperate phage 16-3 of Rhizobium meliloti 41

Autor: Erzsébet Jónás, Anikó Páy, Géza Dallmann, László Orosz, Nándor Garamszegi, Gábor Polner, Zoltán Ascher, László Dorgai
Rok vydání: 1983
Předmět:
Zdroj: Molecular and General Genetics MGG. 191:430-433
ISSN: 1432-1874
0026-8925
DOI: 10.1007/bf00425759
Popis: Restriction cleavage maps for enzymes EcoRI, BamHI, PstI, PvuII, XbaI and EcoRV of Rhizobium meliloti temperate phage 16-3 have been established. Together with the earlier maps (HindIII, KpnI, HpaI, BglII) 98 restriction sites, 'evenly' distributed, have been mapped along the phage genome, including the so far unmarked silent region of the chromosome. All the restriction maps have been fitted to each other by computer optimalization. Beyond for conventional techniques a computer program (PMAP) for physical mapping of linear DNA has been employed which made the experimentation, in several cases, extremely efficient.
Databáze: OpenAIRE