The detailed physical map of the temperate phage 16-3 of Rhizobium meliloti 41
Autor: | Erzsébet Jónás, Anikó Páy, Géza Dallmann, László Orosz, Nándor Garamszegi, Gábor Polner, Zoltán Ascher, László Dorgai |
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Rok vydání: | 1983 |
Předmět: | |
Zdroj: | Molecular and General Genetics MGG. 191:430-433 |
ISSN: | 1432-1874 0026-8925 |
DOI: | 10.1007/bf00425759 |
Popis: | Restriction cleavage maps for enzymes EcoRI, BamHI, PstI, PvuII, XbaI and EcoRV of Rhizobium meliloti temperate phage 16-3 have been established. Together with the earlier maps (HindIII, KpnI, HpaI, BglII) 98 restriction sites, 'evenly' distributed, have been mapped along the phage genome, including the so far unmarked silent region of the chromosome. All the restriction maps have been fitted to each other by computer optimalization. Beyond for conventional techniques a computer program (PMAP) for physical mapping of linear DNA has been employed which made the experimentation, in several cases, extremely efficient. |
Databáze: | OpenAIRE |
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