Mercuric reductase activity and evidence of broad-spectrum mercury resistance among clinical isolates of rapidly growing mycobacteria
| Autor: | Richard J. Wallace, Yijun Pang, V. A. Steingrube, L C Steele |
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| Rok vydání: | 1991 |
| Předmět: |
Hot Temperature
Microorganism Microbial Sensitivity Tests Microbiology Mycobacterium Humans Pharmacology (medical) Mercury(II) reductase Pharmacology chemistry.chemical_classification Mercury Radioisotopes Mycobacterium Infections biology Chemistry Drug Resistance Microbial Mercury biology.organism_classification Enzyme assay Infectious Diseases Enzyme Biochemistry Conjugation Genetic biology.protein Mycobacterium fortuitum Transformation Bacterial Phenylmercuric Acetate Oxidoreductases Bacteria Research Article |
| Zdroj: | Antimicrobial agents and chemotherapy. 35(5) |
| ISSN: | 0066-4804 |
| Popis: | Resistance to mercury was evaluated in 356 rapidly growing mycobacteria belonging to eight taxonomic groups. Resistance to inorganic Hg2+ ranged from 0% among the unnamed third biovariant complex of Mycobacterium fortuitum to 83% among M. chelonae-like organisms. With cell extracts and 203Hg(NO3)2 as the substrate, mercuric reductase (HgRe) activity was demonstrable in six of eight taxonomic groups. HgRe activity was inducible and required NADPH or NADH and a thiol donor for optimai activity. Species with HgRe activity were also resistant to organomercurial compounds, including phenylmercuric acetate. Attempts at intraspecies and intragenus transfer of HgRe activity by conjugation or transformation were unsuccessful. Mercury resistance is common in rapidly growing mycobacteria and appears to function via the same inducible enzyme systems already defined in other bacterial species. This system offers potential as a strain marker for epidemiologic investigations and for studying genetic systems in rapidly growing mycobacteria. |
| Databáze: | OpenAIRE |
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