YKL-40/CHI3L1 facilitates migration and invasion in HER2 overexpressing breast epithelial progenitor cells and generates a niche for capillary-like network formation
Autor: | Jennifer A. Kricker, Sarah Sophie Steinhäuser, Gunnhildur Asta Traustadottir, Saevar Ingthorsson, Zuzana Budkova, Aileen Krueger, Thorarinn Gudjonsson, Erika Morera |
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Přispěvatelé: | Læknadeild (HÍ), Faculty of Medicine (UI), Biomedical Center (UI), Lífvísindasetur (HÍ), Heilbrigðisvísindasvið (HÍ), School of Health Sciences (UI), Háskóli Íslands, University of Iceland |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Epithelial-Mesenchymal Transition Angiogenesis Receptor ErbB-2 Cell Culture Techniques Biology Article Metastasis Transcriptome 03 medical and health sciences 0302 clinical medicine Cell Movement Brjóstakrabbamein Krabbameinsrannsóknir medicine Human Umbilical Vein Endothelial Cells Humans Epithelial–mesenchymal transition Chitinase-3-Like Protein 1 Progenitor cell Mammary Glands Human Migration Cell Proliferation Gen Neovascularization Pathologic Gene Expression Profiling Stem Cells Epithelial to mesenchymal transition (EMT) Cancer Epithelial Cells Cell Biology General Medicine medicine.disease Isogenic human disease models Frumulíffræði 030104 developmental biology 030220 oncology & carcinogenesis Gene Knockdown Techniques Cancer research Female Stem cell YKL-40/CHI3L1 Developmental Biology Invasive breast cancer |
Zdroj: | In Vitro Cellular & Developmental Biology. Animal |
ISSN: | 1543-706X |
Popis: | Publisher's version (útgefin grein). Epithelial to mesenchymal transition (EMT) is a developmental event that is hijacked in some diseases such as fibrosis and cancer. In cancer, EMT has been linked to increased invasion and metastasis and is generally associated with a poor prognosis. In this study, we have compared phenotypic and functional differences between two isogenic cell lines with an EMT profile: D492M and D492HER2 that are both derived from D492, a breast epithelial cell line with stem cell properties. D492M is non-tumorigenic while D492HER2 is tumorigenic. Thus, the aim of this study was to analyze the expression profile of these cell lines, identify potential oncogenes, and evaluate their effects on cellular phenotype. We performed transcriptome and secretome analyses of D492M and D492HER2 and verified expression of selected genes at the RNA and protein level. One candidate, YKL-40 (also known as CHI3L1), was selected for further studies due to its differential expression between D492M and D492HER2, being considerably higher in D492HER2. YKL-40 has been linked to chronic inflammation diseases and cancer, yet its function is not fully understood. Knock-down experiments of YKL-40 in D492HER2 resulted in reduced migration and invasion as well as reduced ability to induce angiogenesis in an in vitro assay, plus changes in the EMT-phenotype. In summary, our data suggest that YKL-40 may provide D492HER2 with increased aggressiveness, supporting cancer progression and facilitating angiogenesis. This work was supported by Grants from Landspitali University Hospital Science Fund, University of Iceland Research Fund, and Icelandic Science and Technology Policy—Grant of Excellence: 152144051. ‘Göngum saman,’ a supporting group for breast cancer research in Iceland ( www.gongumsaman.is ). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
Databáze: | OpenAIRE |
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