Effect of two pyrimidine analogs on accumulation of tubulin in NHIK 3025 cells
| Autor: | Niels O. Juul, Reidar Oftebro, Mina E. Holm Juul, John M. Dornish, Erik O. Pettersen |
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| Rok vydání: | 1990 |
| Předmět: |
Clinical Biochemistry
Mitosis macromolecular substances Sulfone Flow cytometry Cell Line Pyrimidine analogue chemistry.chemical_compound Tubulin medicine Animals Humans Molecular Biology Total protein Brain Chemistry biology medicine.diagnostic_test Sulfoxide Cell Biology General Medicine Molecular biology Rats Pyrimidines chemistry Biochemistry Cell culture biology.protein |
| Zdroj: | Molecular and cellular biochemistry. 96(2) |
| ISSN: | 0300-8177 |
| Popis: | Accumulation of tubulin as compared with the accumulation of total cellular protein in human NHIK 3025 cells treated with the sulfone 2-(2-thenyl)sulfonyl-5-bromopyrimidine (NY 4137) and the sulfoxide 2-(2-thenyl)sulfinyl-5-bromopyrimidine (NY 4138), two mitotic inhibitors, were investigated by two-parametric flow cytometry. Following a 4 h treatment with NY 4137 tubulin accumulation is inhibited while total protein continues to accumulate. After treatment for 4 h with NY 4138 the accumulation of total protein is approximately constant, while the accumulation of tubulin is reduced although not to the same degree as that found for NY 4137-treated cells. In addition, the percentage tubulin SH-groups (6.89 +/- 0.14) remaining after treatment of purified rat brain tubulin with NY 4137 or NY 4138 was determined. Treatment with 0.0125 mM NY 4137 reduced the number of tubulin SH-groups detectable with dithiobis benzoate or from 6.89 +/- 0.14 before treatment to about 4 after treatment. However, practically all SH-groups of tubulin remain detectable following treatment with the same concentration of NY 4138. From the results described in this report we infer that NY 4137 binds to tubulin SH-groups and that inhibition of tubulin accumulation follows as a secondary effect. |
| Databáze: | OpenAIRE |
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