Citrus leprosis virus N: A New Dichorhavirus Causing Citrus Leprosis Disease
| Autor: | Ricardo Harakava, Aline Daniele Tassi, Renato B. Salaroli, Juliana Freitas-Astúa, Elliot W. Kitajima, Camila Chabi-Jesus, Pedro Luis Ramos-González, Orlene Guerra-Peraza |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Citrus Genome Viral Plant Science 01 natural sciences Genome Plant Viruses law.invention 03 medical and health sciences Cytopathogenic Effect Viral law Animals Citrus leprosis disease Orchid fleck virus Cloning Molecular Phylogeny Polymerase chain reaction Plant Diseases Whole genome sequencing Mites biology Nucleic acid sequence food and beverages biology.organism_classification Virology Plant Leaves 030104 developmental biology RNA Viral Brevipalpus phoenicis VETORES DE DOENÇAS DE PLANTAS Viral disease Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1943-7684 0031-949X |
| DOI: | 10.1094/phyto-02-17-0042-r |
| Popis: | Citrus leprosis (CL) is a viral disease endemic to the Western Hemisphere that produces local necrotic and chlorotic lesions on leaves, branches, and fruit and causes serious yield reduction in citrus orchards. Samples of sweet orange (Citrus × sinensis) trees showing CL symptoms were collected during a survey in noncommercial citrus areas in the southeast region of Brazil in 2013 to 2016. Transmission electron microscopy analyses of foliar lesions confirmed the presence of rod-like viral particles commonly associated with CL in the nucleus and cytoplasm of infected cells. However, every attempt to identify these particles by reverse-transcription polymerase chain reaction tests failed, even though all described primers for the detection of known CL-causing cileviruses and dichorhaviruses were used. Next-generation sequencing of total RNA extracts from three symptomatic samples revealed the genome of distinct, although highly related (>92% nucleotide sequence identity), viruses whose genetic organization is similar to that of dichorhaviruses. The genome sequence of these viruses showed |
| Databáze: | OpenAIRE |
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