Adjusting the Cut-Off and Maximum Pool Size in RT-qPCR Pool Testing for SARS-CoV-2
| Autor: | Raissa Prado Rocha, Elaine Leandro Machado, Murilo Soares Costa, Santuza M. R. Teixeira, Ricardo H. C. Takahashi, Flávio Guimarães da Fonseca, Alex F. Carvalho, Hugo Itaru Sato, Unaí Tupinambás, Nathalia Sernizon Guimarães, Claudia Regina Lindgren Alves |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Adult Male 2019-20 coronavirus outbreak Coronavirus disease 2019 (COVID-19) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) 030106 microbiology Sample processing Pooling lcsh:QR1-502 Test sensitivity Real-Time Polymerase Chain Reaction lcsh:Microbiology Article 03 medical and health sciences Young Adult 0302 clinical medicine Virology Statistics Humans 030212 general & internal medicine pool testing Mathematics Aged SARS-CoV-2 COVID-19 RT-qPCR Aged 80 and over Cycle threshold Middle Aged Infectious Diseases COVID-19 Nucleic Acid Testing Female Cut-off |
| Zdroj: | Viruses Viruses; Volume 13; Issue 4; Pages: 557 Viruses, Vol 13, Iss 557, p 557 (2021) |
| ISSN: | 1999-4915 |
| Popis: | Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) to detect SARS-CoV-2 RNA is an essential test to monitor the occurrence of COVID-19. A methodology is proposed for the determination of maximum pool size and adjustments of cut-off values of cycle threshold (Ct in RT-qPCR pool testing, to compensate for the dilution caused by pooling. The trade-off between pool size and test sensitivity is stated explicitly. The procedure was designed to ensure that samples that would be detectable in individual testing remain detectable in pool testing. The proposed relaxation in cut-off is dependent on the pool size, allowing a relatively tight correction to avoid loss of detection of positive samples. The methodology was evaluated in a study of pool testing of adults attending a public emergency care unit, reference for COVID-19 in Belo Horizonte, Brazil, and presenting flu-like symptoms. Even samples on the edge of detectability in individual testing were detected correctly. The proposed procedure enhances the consistency of RT-qPCR pool testing by enforcing that the scales of detectability in pool processing and in individual sample processing are compatible. This may enhance the contribution of pool testing to large-scale testing for COVID-19. |
| Databáze: | OpenAIRE |
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