Identification of proteins and developmental expression of RNAs encoded by the 65A cuticle protein gene cluster in Drosophila melanogaster
| Autor: | Jean-Philippe Charles, Lynn M. Riddiford, Shamim Nejad, Carol Chihara |
|---|---|
| Přispěvatelé: | Développement et Communication Chimique chez les Insectes ( DCCI ), Centre National de la Recherche Scientifique ( CNRS ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Delon, Viviane |
| Rok vydání: | 1998 |
| Předmět: |
Drosophila melanogaster/*genetics/growth & development
Cuticle Molecular Sequence Data Insect Proteins/*genetics/isolation & purification Sequence Homology Genes Insect Larva/genetics/growth & development Biochemistry Gene cluster Animals Developmental Amino Acid Sequence Molecular Biology Gene Genetics Regulation of gene expression Sequence Homology Amino Acid biology fungi Gene Expression Regulation Developmental Chromosome Mapping biology.organism_classification Amino Acid Drosophila melanogaster Gene Expression Regulation Genes Larva Multigene Family Insect Science Ecdysis RNA/*genetics Insect Proteins RNA Instar Insect Overlapping gene |
| Zdroj: | Insect Biochemistry and Molecular Biology Insect Biochemistry and Molecular Biology, Elsevier, 1998, 28 (3), pp.131-8 |
| ISSN: | 0965-1748 |
| DOI: | 10.1016/s0965-1748(97)00107-0 |
| Popis: | 0965-1748 (Print) Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.; Proteins of the third instar larval cuticle of Drosophila melanogaster, LCP5-LCP9, were purified and their N-terminal sequences determined. Three of these proteins (LCP5, 6, and 8) were found to be encoded by two multicopy genes previously mapped to the gene cluster at 65A 5-6 on the left arm of the third chromosome. The analysis of the patterns of developmental expression of the 8 distinct genes at this site showed that all but two were expressed during larval life. The patterns fell into three groups: one where expression was all through larval life, one where expression was primarily in the third instar, and one only during the production of the adult cuticle. One duplicated gene was not expressed in the Canton S strain at any time from the embryo to adult ecdysis. These findings indicate that there is not a unique set of cuticle proteins in the third versus the first and second instar larval cuticles and indicates that overlapping gene sets in several different gene clusters encode the proteins of the cuticle of different developmental stages. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect