Oxalyl-CPG: a labile support for synthesis of sensitive oligonucleotide derivatives
| Autor: | Robert L. Letsinger, Charles N. Singman, Rushdi H. Alul, Guangrong Zhang |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
chemistry.chemical_classification
Oxalates Base Sequence Base (chemistry) Oligonucleotide Molecular Sequence Data Diisopropylamine Biology Combinatorial chemistry Chemical synthesis chemistry.chemical_compound Ammonium hydroxide Oligodeoxyribonucleotides chemistry Biochemistry Covalent bond Genetics Glass Polystyrene Oxidation-Reduction Triethylamine Chromatography High Pressure Liquid Dinucleoside Phosphates |
| Zdroj: | Nucleic Acids Research. 19:1527-1532 |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | A procedure is described for linking nucleosides covalently to controlled pore glass or cross-linked polystyrene supports by means of an oxalyl anchor. Though stable to triethylamine and diisopropylamine, the nucleoside-oxalyl link can be cleaved within a few minutes at room temperature with ammonium hydroxide in methanol. This new anchor can be used in automated synthesis of conventional oligonucleotides. The primary value, however, is that it enables one to employ solid support methodology to synthesize a variety of base-sensitive oligonucleotide derivatives, as illustrated here by synthesis of oligomers with base protecting groups intact and with methyl phosphotriester groups at the internucleoside links. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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