Expression and Function of Apoptosis Initiator FOXO3 in Granulosa Cells During Follicular Atresia in Pig Ovaries
| Autor: | Noboru Manabe, Takafumi Sai, Yasufumi Goto, Akihisa Maeda, Fuko Matsuda, Kazuhiro Sakamaki, Hiroshi Gonda, Naoko Inoue, Yuan Cheng |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
endocrine system
medicine.medical_specialty Fas Ligand Protein Granulosa cell media_common.quotation_subject Follicular Atresia Molecular Sequence Data Sus scrofa Apoptosis Ovary Biology Cell Line Andrology Ovarian Follicle Proto-Oncogene Proteins Internal medicine Follicular phase medicine Animals Humans Protein Isoforms Amino Acid Sequence RNA Messenger Sexual Maturation Ovulation media_common Granulosa Cells Bcl-2-Like Protein 11 Sequence Homology Amino Acid Follicular atresia Forkhead Box Protein O3 Membrane Proteins Forkhead Transcription Factors medicine.disease Endocrinology medicine.anatomical_structure Gene Expression Regulation Organ Specificity Cell culture Atresia FOXO3 Female Animal Science and Zoology Apoptosis Regulatory Proteins Sequence Alignment |
| Zdroj: | Journal of Reproduction and Development. 57:151-158 |
| ISSN: | 1348-4400 0916-8818 |
| DOI: | 10.1262/jrd.10-124h |
| Popis: | In mammalian ovaries, most follicles are lost by atresia before ovulation. It has become apparent that the apoptosis of granulosa cells induces follicular atresia. Forkhead box O3 (FOXO3), also called FKHRL1 (forkhead in rhabdomyosarcoma-like 1), is a proapoptotic molecule that belongs to the FOXO subfamily of forkhead transcription factors. Foxo3-deficient female mice were reported to be infertile because of abnormal ovarian follicular development, but the precise influences of FOXO3 on follicular atresia of mature ovary have not been determined. Therefore, we examined the expression and function of FOXO3 in porcine ovarian follicles and granulosa-derived cells. FOXO3 mRNA levels in granulosa cells of porcine ovaries increased during atresia, while FOXO3 protein was abundant in granulosa cells of early atretic follicles. By immunohistochemistry, the inner surface area of the granulosa layer in early atretic follicles was strongly stained with anti-FOXO3 antibody. The granulosa cells expressing FOXO3 coincided with apoptotic cells, indicating a role of FOXO3 as a proapoptotic factor in granulosa cells of porcine ovaries. In porcine (JC-410) and human (KGN) granulosa-derived cells, cell death was induced by transfection of FOXO3 expression vectors. Expression of the proapoptotic factors Fas ligand (FASLG) and BCL2-like 11 (BCL2L11) was upregulated by FOXO3 in KGN cells. In conclusion, FOXO3 is expressed in porcine ovarian follicles and induces apoptosis in granulosa cells, suggesting that it is a candidate for the initiator of follicular atresia. |
| Databáze: | OpenAIRE |
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