LncRNA TUG1 facilitates proliferation, invasion and stemness of ovarian cancer cell via miR ‐186‐5p/ ZEB1 axis
Autor: | Fu-Liang Zhan, Mei-Zhen Yao, Chun-Fang Chen |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Clinical Biochemistry Cell Biology Biochemistry 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cancer stem cell Cell Line Tumor medicine Humans Gene silencing RNA Neoplasm Cell Proliferation Ovarian Neoplasms Cell growth Zinc Finger E-box-Binding Homeobox 1 Cell Biology General Medicine medicine.disease Neoplasm Proteins Blot MicroRNAs 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Cancer cell Neoplastic Stem Cells Cancer research Female RNA Long Noncoding Ovarian cancer Signal Transduction |
Zdroj: | Cell Biochemistry and Function. 38:1069-1078 |
ISSN: | 1099-0844 0263-6484 |
Popis: | LncRNA TUG1 has been rarely studied in ovarian cancer (OC), our objective was to explore the role of TUG1 in the regulation of malignant phenotypes of OC. Vectors of sh-TUG1, miR-186-5p and pcDNA-ZEB1 were, respectively, constructed and used to infect OC cells. MTT and transwell assays were applied for representing cell proliferation and invasion, respectively. Sphere formation experiment was used to detect the stemness of OC cells. Western blotting and qRT-PCR were employed for detecting the expression of multiple biomarkers on protein and RNA levels, respectively. The luciferase assay was performed to reveal the interactions between miR-186-5p and TUG1 or ZEB1. The silencing of TUG1 and upregulation of miR-186-5p both suppressed the cell proliferation, invasion and cancer stem cell (CSC) properties. Additionally, luciferase assay verified that miR-186-5p directly binds TUG1 and ZEB1. Moreover, overexpression of ZEB1 rescued the impact on the proliferation, invasion and stemness of TUG1 silencing in OC. TUG1 sponges miR-186-5p to release ZEB1 and promotes the proliferation, invasion and stemness of OC cells, suggesting that TUG1 could be a potential therapeutic target for OC therapy. SIGNIFICANCE OF THE STUDY: LncRNA TUG1 could promote proliferation, invasion and stemness of ovarian cancer cells. Our study first discovered that TUG1 play a tumourigenic role in ovarian cancer by regulating stemness of cancer cells. Mechanism research exhibited the regulation role of TUG1 in ovarian cancer cells was miR-186-5p/ZEB1 axis depended. These results provided a new perspective to understand the pathogenesis and development of ovarian cancer; it will offer new evidence for better diagnosis and treatment therapy of ovarian cancer. |
Databáze: | OpenAIRE |
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