Effects of an enamel matrix derivative on human osteoblasts and PDL cells grown in organoid cultures
| Autor: | Stefan Hägewald, Nicole Pischon, Jean-Pierre Bernimoulin, Bernd Zimmermann |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Periodontal Ligament chemistry.chemical_element Calcium Organ Culture Techniques Dental Enamel Proteins Enamel matrix derivative medicine Organoid Humans Periodontal fiber General Dentistry Cells Cultured Cell Proliferation Osteoblasts Infant Osteoblast Anatomy Cell cycle Alkaline Phosphatase Tooth enamel Cell biology medicine.anatomical_structure Bromodeoxyuridine Otorhinolaryngology chemistry Cell culture Microscopy Electron Scanning Proline-Rich Protein Domains Surgery Oral Surgery Peptides Tooth Calcification |
| Zdroj: | Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 102:551-557 |
| ISSN: | 1079-2104 |
| Popis: | The objective of this study was to investigate cellular effects of enamel matrix derivative (EMD) in human derived, primary osteoblasts and periodontal ligament (PDL) cells grown in organoid cultures.Cell replication was assessed by BrdU-incorporation. [(3)H]-proline incorporation was measured to determine the synthesis of proline-containing proteins, such as collagen. In addition, calcium accumulation and alkaline-phosphatase-activity were quantified. Electron microscopy for morphological analysis was performed.Our results showed that EMD enhances BrdU-incorporation in PDL cells and osteoblasts. Also, in osteoblast organoid cultures [3H]-proline incorporation was 3-fold increased (P.01). Extensive matrix deposition was noted in osteoblast cultures by electron microscopy. In osteoblasts, high levels of calcium accumulation and alkaline-phosphatase-activity were found. However, EMD did not promote mineralization.Our results indicate that under organoid culture conditions EMD is able to promote the synthesis of proline-containing proteins such as collagen but not matrix mineralization of primary human osteoblastic cells. |
| Databáze: | OpenAIRE |
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