Cell-free protein synthesis with sodium dodecyl sulfate and ADP
| Autor: | M. Töpert, D.H. Rammler |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Peptide Biosynthesis
Uracil Nucleotides Phenylalanine Polynucleotides Biophysics Biochemistry Phosphoenolpyruvate Surface-Active Agents chemistry.chemical_compound Bacterial Proteins Drug Stability Escherichia coli Methods Chemical Precipitation Sodium dodecyl sulfate Molecular Biology Carbon Isotopes Cell-free protein synthesis Chromatography Cell-Free System Adenine Nucleotides Osmolar Concentration Sodium Dodecyl Sulfate Cell Biology Hydrogen-Ion Concentration Adenosine Diphosphate Kinetics chemistry RNA Fatty Alcohols |
| Zdroj: | Analytical Biochemistry. 48:292-299 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(72)90192-3 |
| Popis: | A procedure is described for increasing the amount of acid-precipitable polyphenylalanine obtained from crude S-30 extracts of E. coli MRE-600 using the poly U directed Nirenberg assay system. The method requires addition of SDS and ADP to the assay solution in addition to its normal constituents. Using this procedure, a 9- to 10-fold increase in acid-precipitable radioactivity is obtained in a 60 min assay period. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|