Diabetic db/db mice exhibit central nervous system and peripheral molecular alterations as seen in neurological disorders
| Autor: | Khalid M. Elased, Hassan Rahmoune, Agnes Ernst, Sabine Bahn, Ajaykumar N. Sharma, Paul C. Guest |
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| Přispěvatelé: | Neurosciences |
| Rok vydání: | 2013 |
| Předmět: |
Central Nervous System
Male Proteomics medicine.medical_specialty Psychosis medicine.medical_treatment Central nervous system Biology Hippocampus Gas Chromatography-Mass Spectrometry Diabetes Mellitus Experimental Mice 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine SDG 3 - Good Health and Well-being Internal medicine Diabetes mellitus medicine Animals Insulin Biological Psychiatry 030304 developmental biology Immunoassay 0303 health sciences diabetes Leptin biomarkers db/db mouse medicine.disease Mice Mutant Strains Frontal Lobe pathway analysis 3. Good health Mice Inbred C57BL Disease Models Animal Psychiatry and Mental health Db/db Mouse psychiatric disorders medicine.anatomical_structure Endocrinology Peptide YY Original Article Psychopharmacology 030217 neurology & neurosurgery |
| Zdroj: | Translational Psychiatry, 3. Nature Publishing Group Translational Psychiatry |
| ISSN: | 2158-3188 |
| DOI: | 10.1038/tp.2013.42 |
| Popis: | The db/db mouse is a widely used preclinical model in diabetes research. Recent studies have shown that these mice also display aspects of psychosis and depression-like behaviors as seen in some psychiatric disorders. Here, we have performed multiplex immunoassay and liquid chromatography mass spectrometry profiling of the plasma and brain samples from db/db and control mice to identify altered pathways, which could be related to these behavioral abnormalities. This is the first study to carry out profiling of the brain proteome in this model. Plasma from the db/db mice had increased levels of leptin and insulin, decreased levels of peptide YY, glucagon and prolactin and alterations in inflammation-related proteins, compared with control mice. Frontal cortex tissue from the db/db mice showed changes in proteins involved in energy metabolism, cellular structure and neural functioning, and the hippocampus had changes in proteins involved in the same pathways, with additional effects on cellular signalling proteins. The overlap of these findings with effects seen in type 2 diabetes, schizophrenia, major depressive disorder and Alzheimer's disease might contribute to a common endophenotype seen in metabolic and neurological disorders. |
| Databáze: | OpenAIRE |
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