Investigation of cell adhesion in chitosan membranes for peripheral nerve regeneration
| Autor: | João F. Mano, Joaquim M. Oliveira, Thomas Freier, Joana M. Silva, Tiago H. Silva, Joana Silva-Correia, Rita López-Cebral, Rui L. Reis, Cristiana R. Carvalho |
|---|---|
| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Materials science Neurite Biocompatibility Bioengineering Nanotechnology 02 engineering and technology Biomaterials Mice 03 medical and health sciences Tissue engineering Cell Adhesion medicine Animals Schwann cells Peripheral Nerves Cell adhesion Fibroblast Cytoskeleton Cell Line Transformed Cell Proliferation Chitosan Science & Technology L929 mouse fibroblast cells Degree of acetylation Biomaterial Membranes Artificial 021001 nanoscience & nanotechnology Nerve Regeneration 3. Good health 030104 developmental biology medicine.anatomical_structure Membrane Mechanics of Materials Biophysics Peripheral nerve injuries Chitosan Degree of acetylation 0210 nano-technology |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | Peripheral nerve injuries have produced major concerns in regenerative medicine for several years, as the recovery of normal nerve function continues to be a significant clinical challenge. Chitosan (CHT), because of its good biocompatibility, biodegradability and physicochemical properties, has been widely used as a biomaterial in tissue engineering scaffolding. In this study, CHT membranes were produced with three different Degrees of Acetylation (DA), envisioning its application in peripheral nerve regeneration. The three CHT membranes (DA I: 1%, DA II: 2%, DA III: 5%) were extensively characterized and were found to have a smooth and flat surface, with DA III membrane having slightly higher roughness and surface energy. All the membranes presented suitable mechanical properties and did not show any signs of calcification after SBF test. Biodegradability was similar for all samples, and adequate to physically support neurite outgrowth. The in vitro cell culture results indicate selective cell adhesion. The CHT membranes favoured Schwann cells invasion and proliferation, with a display of appropriate cytoskeletal morphology. At the same time they presented low fibroblast infiltration. This fact may be greatly beneficial for the prevention of fibrotic tissue formation, a common phenomenon impairing peripheral nerve regeneration. The great deal of results obtained during this work permitted to select the formulation with the greatest potential for further biological tests. This work has received funding from the European Community's Seventh Framework Programme (FP7-HEALTH-2011) under grant agreement no 278612 (BIOHYBRID). This study was also funded by European Union's FP7 Programme under grant agreement no REGPOT-CT2012-316331-POLARIS.The authors thank the chitosan raw material provided by Altakitin S.A., (Lisboa, Portugal). We are further thankful to Silke Fischer, Natascha Heidrich, Kerstin Kuhlemann, Jennifer Metzen, Hildegard Streich and Maike Wesemann (all from the Institute of Neuroanatomy, Hannover Medical School) for their technical support. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|