Identification of Taxreb107 as a lactogenic hormone responsive gene in mammary epithelial cells
| Autor: | Geoffrey J. Lindeman, Kate D. Sutherland, Jane E. Visvader, Sergio Wittlin |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Taxreb107
Mammary gland differentiation Cellular differentiation Mammary gland Lactogenic Biology Representational difference analysis Ribosomal L6 protein Cell Line Mice Mammary Glands Animal Glucocorticoid Lactation Gene expression medicine Animals RNA Messenger Molecular Biology Regulation of gene expression Subtractive hybridisation Caseins Cell Differentiation Epithelial Cells Cell Biology Molecular biology Prolactin DNA-Binding Proteins medicine.anatomical_structure Gene Expression Regulation Hormone |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1642(3):139-147 |
| ISSN: | 0167-4889 |
| DOI: | 10.1016/s0167-4889(03)00121-6 |
| Popis: | Mammary gland development and differentiation is regulated by a number of growth factors and hormones. Milk protein gene expression represents a hallmark of functional mammary epithelial differentiation and is coordinated by the lactogenic hormone prolactin and glucocorticoids. To date, few ‘early-response’ genes transcriptionally activated by lactogenic hormones have been described. We have used representational difference analysis (RDA) to search for lactogenic-responsive genes in SCp2 mouse mammary epithelial cells. One of the cDNAs identified encoded the DNA-binding protein Taxreb107, originally identified as a HTLV-I Tax responsive element binding protein. Increased Taxreb107 expression was confirmed following prolactin and dexamethasone-induced differentiation of SCp2 and HC11 mammary epithelial cells. Taxreb107 RNA levels were developmentally regulated in the mouse mammary gland, where levels increased substantially during mid- and late pregnancy and persisted during lactation. Overexpression of an antisense Taxreb107 cDNA construct or antisense oligonucleotide in HC11 mammary epithelial cells attenuated milk protein gene expression following prolactin and dexamethasone treatment. These findings indicate a role for Taxreb107 as a lactogenic hormone-responsive gene during differentiation of the mammary gland. |
| Databáze: | OpenAIRE |
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