Protein Tyrosine Kinase Activity in T Lymphocytes from Patients with Systemic Lupus Erythematosus
| Autor: | Veronika Brundula, Ana M. Blasini, Martin A. Rodriguez, Liliana Rivas, Susana Salazar, I L Stekman, Magdalena Paris |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adult
Male medicine.medical_specialty animal structures CD3 Complex T-Lymphocytes T cell CD3 Immunology Receptors Antigen T-Cell Syk Lymphocyte Activation Proto-Oncogene Proteins c-fyn FYN Proto-Oncogene Proteins Internal medicine medicine Humans Lupus Erythematosus Systemic Immunology and Allergy ZAP-70 Protein-Tyrosine Kinase Lupus erythematosus biology business.industry ZAP70 hemic and immune systems Protein-Tyrosine Kinases medicine.disease medicine.anatomical_structure Endocrinology Lymphocyte Specific Protein Tyrosine Kinase p56(lck) Case-Control Studies Cancer research biology.protein Female business Tyrosine kinase Signal Transduction Proto-oncogene tyrosine-protein kinase Src |
| Zdroj: | Journal of Autoimmunity. 11:387-393 |
| ISSN: | 0896-8411 |
| Popis: | We have recently observed an abnormal pattern of protein tyrosine phosphoryl-ation in resting T lymphocytes obtained from peripheral blood of patients with systemic lupus erythematosus (SLE). To examine whether these findings may be related to dysregulated protein tyrosine kinase (PTK) function, we tested the relative amount and enzyme activity of the main PTKs involved in the earliest signalling steps triggered via the CD3 pathway. Cell lysates from peripheral blood T cells in SLE patients showed lower amounts of p59(fyn) and p56(lck) as shown by immunoblot. In contrast, the amount of ZAP-70, a PTK of the syk family, was comparable in both groups. However, p59(fyn) immuno-precipitates obtained from unstimulated peripheral blood SLE T cells showed enhanced PTK activity as compared to controls, whereas the PTK activity of p56(lck) and ZAP-70 molecules was comparable in both groups. The unchecked activity of the TCR/CD3-associated src kinase p59(fyn) may alter the balance needed for regulated T cell responses in SLE patients. |
| Databáze: | OpenAIRE |
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