Experimental evidence of recombination in murine noroviruses
| Autor: | Dominique Ziant, Etienne Thiry, Elisabeth Mathijs, Thomas Delwiche, Axel Mauroy, Benoît Muylkens |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Genotype
Molecular Sequence Data ved/biology.organism_classification_rank.species Sequence Homology Viral Plaque Assay Biology medicine.disease_cause Genetic recombination Genome Cell Line law.invention Mice law Virology medicine Animals Cluster Analysis Genotyping Recombination Genetic Genetics Microbial Viability Virulence Reverse Transcriptase Polymerase Chain Reaction ved/biology Macrophages Norovirus Sequence Analysis DNA Recombinant DNA RNA Viral Homologous recombination Recombination Murine norovirus |
| Zdroj: | Journal of General Virology. 91:2723-2733 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/vir.0.024109-0 |
| Popis: | Based on sequencing data, norovirus (NoV) recombinants have been described, but no experimental evidence of recombination in NoVs has been documented. Using the murine norovirus (MNV) model, we investigated the occurrence of genetic recombination between two co-infecting wild-type MNV isolates in RAW cells. The design of a PCR-based genotyping tool allowed accurate discrimination between the parental genomes and the detection of a viable recombinant MNV (Rec MNV) in the progeny viruses. Genetic analysis of Rec MNV identified a homologous-recombination event located at the ORF1-ORF2 overlap. Rec MNV exhibited distinct growth curves and produced smaller plaques than the wild-type MNV in RAW cells. Here, we demonstrate experimentally that MNV undergoes homologous recombination at the previously described recombination hot spot for NoVs, suggesting that the MNV model might be suitable for in vitro studies of NoV recombination. Moreover, the results show that exchange of genetic material between NoVs can generate viruses with distinct biological properties from the parental viruses. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|