Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes
| Autor: | Andrew M. Kropinski, Luiz Francisco Zafalon, Janet I. MacInnes, Alessandro M. Varani, Fernando Antonio de Ávila, Saura R. Silva, Lucas José Luduverio Pizauro, Camila Chioda de Almeida |
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| Přispěvatelé: | Universidade Estadual Paulista (UNESP), University of Guelph, Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Operon
Virulence Factors Science Staphylococcus Virulence Biology medicine.disease_cause Microbiology Article Bacterial Proteins medicine Animals Adhesins Bacterial Mastitis Bovine Phylogeny Subclinical infection Regulator gene Multidisciplinary Comparative genomics Biofilm Staphylococcal Infections medicine.disease Mastitis Bacterial adhesin Biofilms Medicine Cattle Female Transcription Factors |
| Zdroj: | Scientific Reports Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) |
| ISSN: | 2045-2322 |
| Popis: | Mastitis is a common and costly disease on dairy farms, commonly caused by Staphylococcus spp. though the various species are associated with different clinical outcomes. In the current study, we performed genomic analyses to determine the prevalence of adhesion, biofilm, and related regulatory genes in 478 staphylococcal species isolated from clinical and subclinical mastitis cases deposited in public databases. The most prevalent adhesin genes (ebpS, atl, pls, sasH and sasF) were found in both clinical and subclinical isolates. However, the ebpS gene was absent in subclinical isolates of Staphylococcus arlettae, S. succinus, S. sciuri, S. equorun, S. galinarum, and S. saprophyticus. In contrast, the coa, eap, emp, efb, and vWbp genes were present more frequently in clinical (vs. subclincal) mastitis isolates and were highly correlated with the presence of the biofim operon (icaABCD) and its transcriptional regulator, icaR. Co-phylogenetic analyses suggested that many of these adhesins, biofilm, and associated regulatory genes could have been horizontally disseminated between clinical and subclinical isolates. Our results further suggest that several adhesins, biofilm, and related regulatory genes, which have been overlooked in previous studies, may be of use for virulence profiling of mastitis-related Staphylococcus strains or as potential targets for vaccine development. |
| Databáze: | OpenAIRE |
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