Increased MicroRNA-146a Levels in Plasma of Patients with Newly Diagnosed Type 2 Diabetes Mellitus
| Autor: | Xia Wang, Frank B. Hu, Zhilei Shan, Jun Liu, Hui Gao, Ping Yao, Ying Rong, Liegang Liu, Wei Bao, Songfan Xia, Xuefeng Yu |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male medicine.medical_specialty lcsh:Medicine Type 2 diabetes Gastroenterology Asian People Internal medicine Diabetes mellitus Blood plasma Humans Medicine lcsh:Science Multidisciplinary Receiver operating characteristic business.industry lcsh:R Case-control study Area under the curve Type 2 Diabetes Mellitus Middle Aged medicine.disease MicroRNAs Real-time polymerase chain reaction Diabetes Mellitus Type 2 ROC Curve Case-Control Studies Immunology lcsh:Q Female Disease Susceptibility business Heme Oxygenase-1 Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 9, p e73272 (2013) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0073272 |
| Popis: | Background MicroRNAs (miRNAs), a class of small non-coding RNAs, are thought to serve as crucial regulators of gene expression. Dysregulated expression of miRNAs has been described in various diseases and may contribute to related pathologic processes. Our aim was to examine circulating miRNA-146a levels in newly diagnosed type 2 diabetes mellitus (new-T2DM) patients from a Chinese Han population. Methodology/Principal Findings Circulating miRNA-146a was extracted from plasma samples of 90 new-T2DM patients and 90 age- and sex-matched controls. Quantitative PCR assessment revealed that circulating miRNA-146a levels were significantly elevated in new-T2DM patients compared with controls. Participants in the highest tertile of circulating miRNA-146a levels showed a notably higher risk for new-T2DM (crude OR 4.333, 95% CI, 1.935 to 9.705, P = 0.001) than persons in the lowest tertile. Controlling for known risk factors and some biochemical indicators did not attenuate the aforementioned association. In addition, receiver operating characteristic (ROC) curves generated for miRNA-146a revealed an area under the curve (AUC) of 0.725 (95% CI, 0.651 to 0.799, P < 0.001). Moreover, higher circulating miRNA-146a levels were significantly associated with higher plasma heme oxygenase-1 (HO-1) concentrations (β coefficient = 0.131, P < 0.001) and lower HOMA-beta (β coefficient = -0.153, P = 0.015). Conclusions/Significance We found that circulating miRNA-146a levels were significantly elevated in new-T2DM patients compared with healthy controls. Whether expression of circulating miRNA-146a holds predictive value for T2DM warrants further investigations. |
| Databáze: | OpenAIRE |
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