A comparative study of the effectiveness of sinus bone grafting with recombinant human bone morphogenetic protein 2–coated tricalcium phosphate and platelet-rich fibrin–mixed tricalcium phosphate in rabbits
| Autor: | Hyun-Su Baek, Bok-Joo Kim, Sang-Hun Shin, Dae-Seok Hwang, Jin-Sook Jeong, In-Kyo Chung, Chul-Hun Kim, Taek-Kyun Kwon |
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| Rok vydání: | 2012 |
| Předmět: |
Blood Platelets
Calcium Phosphates Sinus Floor Augmentation Pathology medicine.medical_specialty Bone Regeneration medicine.medical_treatment Bone Morphogenetic Protein 2 Dentistry Bone healing Bone grafting Bone morphogenetic protein 2 Pathology and Forensic Medicine Masson's trichrome stain Coated Materials Biocompatible Transforming Growth Factor beta Animals Medicine Radiology Nuclear Medicine and imaging Dentistry (miscellaneous) Bone regeneration Drug Carriers Fibrin Bone decalcification business.industry Recombinant Proteins Platelet-rich fibrin Female Surgery Rabbits Oral Surgery business |
| Zdroj: | Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology. 113:583-592 |
| ISSN: | 2212-4403 |
| Popis: | Objectives The objective of this histologic study was to evaluate platelet-rich fibrin (PRF)–mixed tricalcium phosphate (TCP) and recombinant human bone morphogenic protein 2 (rhBMP-2)–coated TCP in their potential to enhance bone regeneration in sinus elevation in rabbits as well as in their inflammatory features. Study Design Bilateral round-shaped defects (diameter 8.0 mm) were formed in the maxillary anterior sinus walls of 36 New Zealand white rabbits. The defects were grafted with TCP only (control group), with rhBMP-2–coated TCP (experimental group A) and with PRF-mixed TCP (experimental group B). Each group included 12 rabbits. The animals were killed at 3 days, 1 week, 2 weeks, 4 weeks, 6 weeks, and 8 weeks. The specimens underwent decalcification and were stained for histologic analysis. Results There were no significant differences in inflammatory features among the groups at 3 days or the first week after operation. In a histomorphometric analysis, the new bone formation ratio showed significant differentiation between groups A and B. The TCP-only control group showed a relatively lower bone formation ratio rather than the experimental groups. The PRF-mixed TCP group showed a larger bone formation area, compared with both the control group and group A. Conclusions In the results of the histologic evaluation (hematoxylin-eosin, Masson trichrome stain), the experimental groups A and B showed rapid bone formation, remodeling, and calcification in the second week. Moreover, there was a significant difference between those experimental groups and the control group in the new bone formation area at the fourth, sixth, and eighth weeks. The PRF-mixed TCP showed more rapid bone healing than the rhBMP-2-coated TCP or the TCP-only control. |
| Databáze: | OpenAIRE |
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