PLD1 promotes dendritic spine morphogenesis via activating PKD1
| Autor: | Li-Da Luo, Tian-Jie Lyu, Zhi-Wen Hu, Cheng Cen, Yun Wang, Li Wenqi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Nervous system TRPP Cation Channels Dendritic spine Immunoprecipitation Dendritic Spines Neurogenesis Dendritic spine morphogenesis Biology Cell Line Rats Sprague-Dawley Mice 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Phospholipase D medicine Animals Molecular Biology Cells Cultured PKD1 Cell Biology Rats 030104 developmental biology medicine.anatomical_structure Excitatory postsynaptic potential Protein kinase D1 Neuroscience 030217 neurology & neurosurgery Phospholipase D1 Protein Binding |
| Zdroj: | Molecular and Cellular Neuroscience. 99:103394 |
| ISSN: | 1044-7431 |
| Popis: | Dendritic spines on the dendrites of pyramidal neurons are one of the most important components for excitatory synapses, where excitatory information exchanges and integrates. The defects of dendritic spine development have been closely connected with many nervous system diseases including autism, intellectual disability and so forth. Based on our previous studies, we here report a new functional signaling link between phospholipase D1 (PLD1) and protein kinase D1 (PKD1) in dendritic spine morphogenesis. Coimmunoprecipitation assays showed that PLD1 associates with PKD1. A series of knocking down and rescuing experiments demonstrated that PLD1 acts upstream of PKD1 in positively regulating dendritic spine morphogenesis. Using PLD1 inhibitor, we found that PLD1 activates PKD1 to promote dendritic spine morphogenesis. Thus, we further reveal the roles of the two different enzymes in neuronal development. |
| Databáze: | OpenAIRE |
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