Microbial analysis and enrichment of anaerobic phenol and p-cresol degrading consortia with addition of AQDS
| Autor: | Xiaoli Su, Yanling Qiu, Yan Xu, Xiaoxia Wang, Xiaojiao Sun, Mingmei Chi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Syntrophus aciditrophicus
Deltaproteobacteria Environmental Engineering cryptanaerobacter phenolicus phenols degradation 0208 environmental biotechnology Anthraquinones 02 engineering and technology 010501 environmental sciences medicine.disease_cause anaerobic syntrophism 01 natural sciences Environmental technology. Sanitary engineering chemistry.chemical_compound Cresols syntrophorhabdus aromaticivorans Biotransformation RNA Ribosomal 16S medicine Phenol Anaerobiosis In Situ Hybridization Fluorescence TD1-1066 0105 earth and related environmental sciences Water Science and Technology biology Chemistry Cresol Biodegradation biology.organism_classification 020801 environmental engineering Biodegradation Environmental Environmental chemistry Peptococcaceae aqds p-Cresol Bacteria medicine.drug |
| Zdroj: | Water Science and Technology, Vol 84, Iss 3, Pp 683-696 (2021) |
| ISSN: | 1996-9732 0273-1223 |
| Popis: | Quinones and humus are ubiquitous in the biosphere and play an important role in the anaerobic biodegradation and biotransformation of organic acids, poisonous compounds as well as inorganic compounds. The impact of humic model compound, anthraquinone-2, 6-disulfonate (AQDS) on anaerobic phenol and p-cresol degradation were studied. Four methanogenic AQDS-free phenol and p-cresol enrichments and two phenol-AQDS enrichments were obtained using two sludges with potential biodegradability of phenol and cresol isomers as inoculum. 16S rRNA gene-cloning analysis combined with fluorescence in situ hybridization revealed that syntrophic aromatic compound degrading bacterium Syntrophorhabdus aromaticivorans was dominant in four AQDS-free enrichments, whereas phenol degrading Cryptanaerobacter phenolicus was dominant in two phenol-AQDS enrichments. Neither co-culture of S. aromaticivorans with Methanospirillum hungatei nor two phenol-AQDS enrichments could metabolize phenol using AQDS as the terminal electron acceptor. Further degradation experiments suggested that C. phenolicus related microbes in two phenol-AQDS enrichments were responsible for the conversion of phenol to benzoate, and benzoate was further degraded by benzoate degraders of Syntrophus aciditrophicus or Sporotomaculum syntrophicum to acetate. HIGHLIGHTS Anaerobic degradation of phenol and p-cresol were faster than o- and m-cresols.; 2 mM AQDS mitigated phenol and completely inhibited p-cresol degradation.; The dominant bacteria in AQDS-free and phenol-AQDS enrichments were different.; Neither S. aromaticivorans nor C. phenolicus affinities could use AQDS as TEA.; The characteristic of key phenol degraders in phenol-AQDS enrichments was described. |
| Databáze: | OpenAIRE |
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