Dengue NS1 and prM antibodies increase the sensitivity of acute dengue diagnosis test and differentiate from Japanese encephalitis infection
| Autor: | V. Thenmozhi, K. J. Dhananjeyan, K Venkatasubramani, Paramasivan R, S. Gowri Sankar, Brij Kishore Tyagi, S. John Vennison, T. Balaji |
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| Rok vydání: | 2014 |
| Předmět: |
viruses
Immunology India Cross Reactions Viral Nonstructural Proteins Antibodies Viral medicine.disease_cause Sensitivity and Specificity Cross-reactivity Virus Dengue fever Dengue Diagnosis Differential Viral Proteins medicine Humans Immunology and Allergy Encephalitis Japanese biology Diagnostic Tests Routine business.industry Outbreak Dengue Virus Japanese encephalitis medicine.disease Case management biology.organism_classification Virology Flavivirus Early Diagnosis Disease Progression biology.protein Antibody business |
| Zdroj: | Journal of Immunological Methods. 407:116-119 |
| ISSN: | 0022-1759 |
| Popis: | Accurate and early diagnosis of dengue infection is essential for dengue case management. In outbreak conditions, it is essential to include two different tests to diagnose dengue and the choice depends on the number of days after the onset of illness in which the sample is collected. During the laboratory diagnosis of dengue in late acute and convalescent phase by MAC-ELISA, it is necessary to rule out possible cross reactions of closely related flavivirus, such as Japanese encephalitis virus which is commonly co-circulating. In the present investigation, the usefulness of dengue virus NS1 and prM antibodies in diagnosing and differentiating dengue from Japanese encephalitis infection was assessed using samples collected during out-breaks. It was shown here that, detection of antibodies against dengue NS1 and prM proteins increases the sensitivity of dengue diagnosis until 15 days. Moreover, detection of antibodies against both proteins was able to differentiate dengue from Japanese encephalitis infection. |
| Databáze: | OpenAIRE |
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