Increased p21-activated kinase-1 expression is associated with invasive potential in uveal melanoma
| Autor: | Nelleke A. Gruis, Wieke Zuidervaart, Frans A. van Nieuwpoort, Sandra Pavey, Nicholas K. Hayward, Martine J. Jager, Leisl M. Packer |
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| Rok vydání: | 2006 |
| Předmět: |
Uveal Neoplasms
Cancer Research Cell type Pathology medicine.medical_specialty Microarray Motility Dermatology Biology Protein Serine-Threonine Kinases Cell Movement medicine Biomarkers Tumor Tumor Cells Cultured Humans Neoplasm Invasiveness Melanoma Oligonucleotide Array Sequence Analysis Matrigel Gene knockdown Gene Expression Profiling DNA Neoplasm medicine.disease Prognosis Phenotype Neoplasm Proteins Gene expression profiling Drug Combinations Oncology p21-Activated Kinases Proteoglycans Collagen Laminin |
| Zdroj: | Melanoma research. 16(4) |
| ISSN: | 0960-8931 |
| Popis: | Prognosis in patients with uveal melanoma is poor as approximately half of all tumors metastasize and currently there are no effective treatments for disseminated disease. Differences in invasiveness between uveal melanomas could therefore be of major significance regarding clinical outcome. To identify genes associated with invasive potential, we have used microarray expression profiling combined with phenotypic characterization of uveal melanoma and melanocyte cell lines to define a gene signature associated with cellular invasion. A panel of 14 uveal cell cultures was assessed using three assays of invasiveness: matrigel invasion chamber system, scratch wound closure and cell motility. We identified a set of 853 differentially expressed transcripts (Wilcoxon–Mann–Whitney test, P |
| Databáze: | OpenAIRE |
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