NCAM: a surface marker for human small cell lung cancer cells
| Autor: | Manfred Gratzl, Martin Rotsch, Maria C. Aletsee-Ufrecht, Keith Langley, Klaus Havemann |
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| Rok vydání: | 1990 |
| Předmět: |
Lung Neoplasms
Cell Adhesion Molecules Neuronal Enolase Biophysics Biology Biochemistry Immunoenzyme Techniques Structural Biology Biomarkers Tumor Tumor Cells Cultured Genetics medicine Humans Carcinoma Small Cell Lung cancer Molecular Biology Cell adhesion molecule Large cell Cell Biology Marker medicine.disease Neural cell adhesion molecule Microscopy Electron nervous system Cell culture Immunology Synaptophysin biology.protein Cancer research Adenocarcinoma |
| Zdroj: | FEBS Letters. 267:295-300 |
| ISSN: | 0014-5793 |
| Popis: | Immunocytochemical and immunochemical techniques were used to study the expression of the neural cell adhesion molecule (NCAM) by human lung cancer cell lines. Intense surface staining for NCAM was found at light and electron microscopic levels on small cell lung cancer cells. The NCAM polypeptide of Mr 140000 (NCAM 140) was detected by immunoblotting in all of 7 small cell lung cancer cell lines examined and in one out of two of the closely related large cell cancer cell lines: it was not detected in cell lines obtained from one patient with a mesothelioma, in two cases of adenocarcinoma, nor in two cases of squamous cell cancer. In contrast, neuron-specific enolase was found by immunoblotting in all the lung cancer cell lines tested and synaptophysin in all but the adenocarcinoma cell lines. These antigens were localized intracellularly. The specific expression of NCAM 140 by human small and large cell lung carcinomas suggests its potential as a diagnostic marker. |
| Databáze: | OpenAIRE |
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