Physiological Involvement in pH Signaling of Vps24-mediated Recruitment of Aspergillus PalB Cysteine Protease to ESCRT-III
| Autor: | Herbert N. Arst, Miguel A. Peñalva, América Hervás-Aguilar, Olga Rodríguez-Galán, Antonio Galindo |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_treatment
Proteolysis Mutant macromolecular substances Biology Biochemistry Aspergillus nidulans Gene Expression Regulation Enzymologic ESCRT Fungal Proteins 03 medical and health sciences Gene Expression Regulation Fungal medicine Molecular Biology Transcription factor 030304 developmental biology 0303 health sciences Fungal protein Protease medicine.diagnostic_test Mechanisms of Signal Transduction 030302 biochemistry & molecular biology Cell Biology Hydrogen-Ion Concentration Cysteine protease 3. Good health Cysteine Endopeptidases Protein Transport Multiprotein Complexes Mutation Signal transduction Signal Transduction Transcription Factors |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname The Journal of Biological Chemistry |
| ISSN: | 0021-9258 |
| Popis: | 10 p.-5 fig Activation of the Aspergillus nidulans transcription factor PacC, which mediates ambient pH regulation of gene expression and is recruited to ESCRT-III by the Vps32-interacting scaffold PalA, involves its ambient pH-dependent C-terminal proteolysis. This reaction is almost certainly catalyzed by the PalB calpain-like protease. Here we show that PalB associates with membranes and interacts specifically and directly with ESCRT-III Vps24. The PalB N-terminal MIT domain and the Vps24 C-terminal MIM motif are necessary and sufficient for this interaction. PalB(DeltaMIT), a mutant PalB lacking the MIT domain is inefficiently recruited to membranes and impaired in PacC proteolytic processing. Notably, membrane recruitment is promoted and PacC processing largely restored by covalent attachment of Vps24 to mutant PalB(DeltaMIT). This is the first reported evidence that calpain-like recruitment to ESCRT-III lattices plays a physiological role. It unambiguously positions the calpain-like protease PalB within the ESCRT-III-associated pH signaling complex, underlines the positive role of ESCRT-III in ambient pH signal transduction, and suggests a possible mechanism for PalB activation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|