Effects of Sphincterotomy and Pudendal Nerve Transection on the Anal Sphincter in a Rat Model
| Autor: | Tracy L. Hull, Robert S. Butler, Paul Zaszczurynski, Levilester Salcedo, Massarat Zutshi, Margot S. Damaser |
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| Rok vydání: | 2009 |
| Předmět: |
Pudendal nerve
Rat model Action Potentials Anal Canal Electromyography Rats Sprague-Dawley Pressure medicine Animals Muscle Strength medicine.diagnostic_test business.industry Anal wink Gastroenterology Reproducibility of Results General Medicine Anatomy Nerve injury Anus Muscle Striated Rats Disease Models Animal medicine.anatomical_structure Anesthesia Sphincter Female medicine.symptom Anal sphincter business Muscle Contraction |
| Zdroj: | Diseases of the Colon & Rectum. 52:1321-1329 |
| ISSN: | 0012-3706 |
| DOI: | 10.1007/dcr.0b013e31819f746d |
| Popis: | Purpose Our objective was to define anal resting pressure and electromyography of the normal rat anal sphincter and investigate the short-term effects of both mechanical trauma to the anal sphincter muscles and pudendal nerve transection. Methods Forty-five virgin female Sprague-Dawley rats were randomly allotted to three groups: controls (n = 21), sphincterotomy (n = 12), and pudendal nerve transection (n = 12). Anal pressure was monitored using a saline-filled balloon connected to a pressure transducer. Anal pressure and electromyography of the anal sphincter with use of a needle electrode were recorded both before and after injury or succinylcholine administration. Results Anal pressure data were consistent with rhythmic pressure contractions. Succinylcholine significantly reduced both pressure and electromyography signals. Electromyography amplitude and frequency decreased after nerve transection but not after sphincterotomy. The histology showed that the rat anal anatomy has muscular components that compare with human anatomy. The sphincterotomy group showed injury to the anal sphincters and the sphincter anatomy of the nerve transection group appeared similar to the control group. The anal pressure wave appears to be created by synergistic activity of both striated and smooth muscle of the anal sphincter. Conclusion The female rat is a suitable and reliable model for studying effect of direct and indirect injury to the anal sphincters. |
| Databáze: | OpenAIRE |
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