Temporal oral microbiome changes with brushing in children with cleft lip and palate
| Autor: | Martin S. Mortensen, António Bessa Monteiro, Paula V. Morais, Søren J. Sørensen, Rowney Furfuro, Maria Helena Fernandes, Maria Conceição Manso, Catia D. Carvalho Silva, Ana P. Chung, Rita Rodrigues |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Children and preteen Genus Rothia Dentistry Alloprevotella 03 medical and health sciences 0302 clinical medicine Age groups Medicine Microbiome lcsh:Social sciences (General) lcsh:Science (General) Leptotrichia Multidisciplinary business.industry Rothia Microbial composition 030104 developmental biology Next-generation sequencing lcsh:H1-99 Oral Microbiome CLP business 030217 neurology & neurosurgery lcsh:Q1-390 Cohort study Research Article |
| Zdroj: | Rodrigues, R, Chung, A P, Mortensen, M S, Fernandes, M H, Monteiro, A B, Furfuro, R, Silva, C C, Manso, M C, Sørensen, S J & Morais, P V 2021, ' Temporal oral microbiome changes with brushing in children with cleft lip and palate ', Heliyon, vol. 7, e06513 . https://doi.org/10.1016/j.heliyon.2021.e06513 Heliyon Heliyon, Vol 7, Iss 3, Pp e06513-(2021) |
| DOI: | 10.1016/j.heliyon.2021.e06513 |
| Popis: | This cohort study aimed to characterize the oral microbiome of children with CLP, from two different age groups, and evaluate the effect of supervised or unsupervised toothbrushing on the microbiome of the cleft over time. Swab samples were collected from the cleft area at three different time points (A; no brushing, B; after 15 days and C; after 30 days) and were analyzed using next-generation sequencing to determine the microbial composition and diversity in these time points. Overall, brushing significantly decreased the abundance of the genera Alloprevotella and Leptotrichia in the two age groups examined, and for Alloprevotella this decrease was more evident for children (2–6 years old). In the preteen group (7–12 years old), a significant relative increase of the genus Rothia was observed after brushing. In this study, the systematic brushing over a period of thirty days also resulted in differences at the intra-individual bacterial richness. microbiome; children; CLP, next-generation sequencing; Alloprevotella; Leptotrichia; Rothia. |
| Databáze: | OpenAIRE |
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